Reilly L M, Guarino L A
Department of Entomology, Texas A&M University, College Station, Texas, 77843-2128, USA.
Virology. 1996 Apr 1;218(1):243-7. doi: 10.1006/viro.1996.0185.
The baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) encodes a protein with significant homology to ubiquitin. To study the role of viral ubiquitin in infection, a recombinant virus was constructed with a frameshift mutation within the coding sequence of the viral ubiquitin gene, v-ubi. This recombinant, named Vubi-FS, was viable, indicating that viral ubiquitin is not essential for replication in tissue culture. However, the yields of infectious budded virus were decreased 5- to 10-fold in single step growth curves, and the production of total budded virions was reduced to a similar extent. The mutant virus particles contained the phospholipid-modified form of ubiquitin (Pt-Ub), and amino acid sequence analysis revealed that only host ubiquitin was packaged into virions. Together, these results suggest that viral ubiquitin is a nonessential protein that may confer a slight growth advantage under certain conditions.
杆状病毒苜蓿银纹夜蛾核型多角体病毒（AcNPV）编码一种与泛素具有显著同源性的蛋白质。为了研究病毒泛素在感染过程中的作用，构建了一种重组病毒，该病毒在病毒泛素基因v-ubi的编码序列内存在移码突变。这种重组病毒名为Vubi-FS，具有活性，这表明病毒泛素对于在组织培养中的复制并非必不可少。然而，在单步生长曲线中，感染性出芽病毒的产量降低了5至10倍，并且总出芽病毒粒子的产生也减少到类似程度。突变病毒粒子含有磷脂修饰形式的泛素（Pt-Ub），氨基酸序列分析表明只有宿主泛素被包装到病毒粒子中。这些结果共同表明，病毒泛素是一种非必需蛋白，在某些条件下可能赋予轻微的生长优势。
