Yang C C, Lin S D, Yu H S
Department of Surgery, Kaohsiung Medical College Hospital, Taiwan.
J Dermatol Sci. 1997 Feb;14(2):162-9. doi: 10.1016/s0923-1811(96)00571-3.
We have examined the effects of four 'exogenous' growth factors, i.e. PDGF-BB (5 ng/ml), TGF-beta1 (5 ng/ml), bFGF (10 ng/ml) and EGF (10 ng/ml) on the contraction of floating collagen type I lattices populated by human normal skin (NS) and hypertrophic scar (HS) fibroblasts (FPCL). Only TGF-beta1 enhanced the contractility of both NS and HS fibroblasts in the collagen lattice (P < 0.01). Other growth factors (PDGF-BB, bFGF and EGF) did not affect FPCLs contraction at 72 h (P > 0.05). The onset effect of TGF-beta1 on NS-FPCL contraction was relative early at 24 h after FPCL casting as compared to a 72 h delay on HS-FPCL contraction. Besides, PDGF-BB was found to be able to enhance HS-FPCL contraction (P < 0.05) but not on NS-FPCL contraction on day 4. On the other hand, three enzyme-linked immunosorbent assays (ELISA) were performed to demonstrate quantitatively the 'endogenous' growth factors that fibroblasts secreted into the culture medium 48 h after FPCL casting. No appreciable difference was found between 10 NS and 11 HS samples tested for PDGF-AB immunoassay (11.48 +/- 5.5 pg/ml versus 12.20 +/- 5.34 pg/ml). The same result existed in 7 NS and 13 HS samples for TGF-beta2 immunoassay (15.15 +/- 6.2 pg/ml versus 11.84 +/- 7.46 pg/ml). In bFGF immunoassay study, relative variable data was noted in both 7 NS (18.18 +/- 13.18 pg/ml) and 12 HS samples (20.41 +/- 22.36 pg/ml). In conclusion, we suppose that TGF-beta role in wound healing may be due to the secondary exogenous influences. The endogenous ability of TGF-beta2 secretion (quantity) in HS fibroblasts are the same as NS fibroblasts but with delayed timing responses (quality) to exogenous TGF-beta1 effect in the collagen lattice. Further studies with timing-regulated selective specific monoclonal antibodies against the growth factor receptors may provide the therapeutic applications on HS during wound healing.
我们研究了四种“外源性”生长因子，即血小板衍生生长因子 - BB（PDGF - BB，5 纳克/毫升）、转化生长因子 - β1（TGF - β1，5 纳克/毫升）、碱性成纤维细胞生长因子（bFGF，10 纳克/毫升）和表皮生长因子（EGF，10 纳克/毫升）对由人正常皮肤（NS）和肥厚性瘢痕（HS）成纤维细胞构成的漂浮 I 型胶原晶格收缩的影响。只有 TGF - β1 增强了胶原晶格中 NS 和 HS 成纤维细胞的收缩能力（P < 0.01）。其他生长因子（PDGF - BB、bFGF 和 EGF）在 72 小时时未影响胶原晶格收缩（P > 0.05）。与 HS - FPCL 收缩延迟 72 小时相比，TGF - β1 对 NS - FPCL 收缩的起始作用在 FPCL 铸造后 24 小时相对较早。此外，发现 PDGF - BB 在第 4 天能够增强 HS - FPCL 收缩（P < 0.05），但对 NS - FPCL 收缩无影响。另一方面，进行了三种酶联免疫吸附测定（ELISA）以定量证明成纤维细胞在 FPCL 铸造后 48 小时分泌到培养基中的“内源性”生长因子。在检测 PDGF - AB 的免疫测定中，10 个 NS 样本和 11 个 HS 样本之间未发现明显差异（11.48 ± 5.5 皮克/毫升对 12.20 ± 5.34 皮克/毫升）。在检测 TGF - β2 的免疫测定中，7 个 NS 样本和 13 个 HS 样本也得到相同结果（15.15 ± 6.2 皮克/毫升对 11.84 ± 7.46 皮克/毫升）。在 bFGF 免疫测定研究中，7 个 NS 样本（18.18 ± 13.18 皮克/毫升）和 12 个 HS 样本（20.41 ± 22.36 皮克/毫升）均记录到相对可变的数据。总之，我们推测 TGF - β 在伤口愈合中的作用可能归因于继发性外源性影响。HS 成纤维细胞中 TGF - β2 的内源性分泌能力（数量）与 NS 成纤维细胞相同，但在胶原晶格中对外源性 TGF - β1 作用的时间反应（质量）延迟。使用针对生长因子受体的时间调节选择性特异性单克隆抗体进行进一步研究可能为伤口愈合期间的 HS 提供治疗应用。
