Allan J C, Velasquez-Tohom M, Torres-Alvarez R, Yurrita P, Garcia-Noval J
Department of Biological Sciences, University of Salford, United Kingdom.
Am J Trop Med Hyg. 1996 Apr;54(4):352-6. doi: 10.4269/ajtmh.1996.54.352.
A microplate-type enzyme-linked immunosorbent assay for the detection of Taenia species antigen in human feces was tested in field studies undertaken in two Guatemalan communities. The test was based on immunoglobulin G antibodies from a rabbit hyperimmunized to Taenia solium proglottides. Comparison was made with microscopy and patient interviews as a means of diagnosis. The coproantigen test result was positive in 79 of the 1,582 fecal samples examined. Parasitologic confirmation was made in 55 of these cases. The coproantigen test was the most sensitive technique used, detecting 2.6 times as many confirmed cases of taeniasis as microscopy, which diagnosed 21 cases. Only one case was detected by interviewing. Microscopy revealed on false-negative coproantigen result. Mass treatment of the population did not result in the detection of any additional cases. Twelve coproantigen-positive results were categorized as unconfirmed and an additional 12 as putative false-positive results, giving an overall specificity of 99.2% for the coproantigen test. Of the 34 taeniid tapeworms identified to the species level, all were T. solium. The practicalities of the use of such a test in epidemiologic studies on human taeniasis are discussed.
一种用于检测人类粪便中绦虫属抗原的微孔板型酶联免疫吸附测定法在危地马拉两个社区进行的现场研究中进行了测试。该测试基于用猪带绦虫节片超免疫的兔子的免疫球蛋白G抗体。与显微镜检查和患者访谈作为诊断手段进行了比较。在所检查的1582份粪便样本中，有79份粪抗原检测结果呈阳性。其中55例得到寄生虫学证实。粪抗原检测是所用方法中最敏感的，检测出的绦虫病确诊病例是显微镜检查的2.6倍，显微镜检查诊断出21例。通过访谈仅检测出1例。显微镜检查发现1例假阴性粪抗原结果。对人群进行大规模治疗未检测到任何其他病例。12份粪抗原阳性结果被归类为未确诊，另有12份被视为可能的假阳性结果，粪抗原检测的总体特异性为99.2%。在鉴定到种水平的34条带绦虫中，均为猪带绦虫。讨论了在人类绦虫病流行病学研究中使用这种检测方法的实用性。
