Institute Gulbenkian de Ciencia. Rua Quinta Grande 6, PO box 14, CP 2780-156 Oeiras, Portugal.
Instituto de Investigaciones Biomédicas "Dr. Francisco J. Triana-Alonso", (BIOMED-UC) Facultad de Ciencias de la Salud, Sede Aragua, Universidad de Carabobo, Av. Las Delicias, Maracay, PO Box: 2351, Aragua, Venezuela; Dpto. Clínico-Integral. Escuela de Bioanálisis, Facultad de Ciencias de la Salud, Sede Aragua, Universidad de Carabobo, Maracay, Venezuela.
Acta Trop. 2020 Jul;207:105445. doi: 10.1016/j.actatropica.2020.105445. Epub 2020 Mar 26.
The objective of this study was to identify and treat carriers of adult Taenia solium present in two rural Venezuelan communities through examination of faecal samples by coproscopical analysis, and by the application of a polyclonal and a monoclonal (VP-1) coproantigen ELISA. Both the polyclonal and monoclonal ELISA's were negative when tested with soluble extracts of adults of Ascaris lumbricoides, Hymenolepis nana and Trichuris trichura. The polyclonal ELISA was positive for soluble extracts adults of T. solium and T. saginata, whereas the monoclonal ELISA, which recognizes a glycoprotein, was restricted to T. solium, and was also negative with faecal samples from five cases of T. saginata adult infections. In the first community studied, Potrero Largo (Total population: 300), of 248 faecal samples examined, 2 individuals were positive for Taenia spp eggs by coproscopical analysis and the VP-1 ELISA, and yielded T. solium adults upon purging. In contrast, when the polyclonal coproAg ELISA was applied to the same 248 faecal samples, there were a considerable number of positives. Indeed, seven patients highly positive in the polyclonal ELISA did not yield a Taenia spp upon purging and were negative in the VP-1 ELISA. In the second community studied La Yuca (Total population 560), none of the 333 individuals who donated faeces was positive for Taenia spp eggs. Many, however, were infected with a range of intestinal helminth and protozoan parasites. A total of 76 faecal samples with representative intestinal parasite were then tested in the polyclonal and VP-1 assays. Of these, many gave an unacceptable number of significant optical densities in the polyclonal coproAg ELISA. In contrast, all were negative in the VP-1 ELISA, thus providing evidence for the species specificity of the VP-1 ELISA in faecal samples. These results with the VP-1 coproAg ELISA, although preliminary, justify further validation through the testing of more faecal samples from T. solium and T. saginata adult infected individuals.
本研究的目的是通过粪便样本的粪便检查，应用多克隆和单克隆（VP-1）粪便抗原 ELISA 检测，来鉴定和治疗两个委内瑞拉农村社区中的成人带绦虫携带者。多克隆和单克隆 ELISA 与蛔虫、细粒棘球绦虫和毛首鞭形线虫成虫的可溶性提取物一起检测时均为阴性。多克隆 ELISA 对猪带绦虫和牛带绦虫成虫的可溶性提取物呈阳性，而单克隆 ELISA 识别糖蛋白，仅对猪带绦虫呈阳性，且对 5 例牛带绦虫成虫感染的粪便样本也呈阴性。在第一个研究的社区 Potrero Largo（总人口：300）中，对 248 份粪便样本进行检查，2 份样本通过粪便镜检和 VP-1 ELISA 检测到带绦虫卵阳性，并通过驱虫获得了猪带绦虫成虫。相比之下，当对相同的 248 份粪便样本应用多克隆 coproAg ELISA 时，有相当数量的阳性结果。事实上，多克隆 ELISA 高度阳性的 7 例患者在驱虫后并未产生带绦虫，并且在 VP-1 ELISA 中呈阴性。在第二个研究的社区 La Yuca（总人口 560）中，没有一个捐献粪便的 333 人对带绦虫卵呈阳性。然而，许多人感染了一系列肠道寄生虫和原生动物寄生虫。然后，对 76 份具有代表性的肠道寄生虫粪便样本进行了多克隆和 VP-1 检测。其中，许多在多克隆 coproAg ELISA 中产生了不可接受数量的显著光密度值。相比之下，所有样本在 VP-1 ELISA 中均为阴性，从而为 VP-1 ELISA 在粪便样本中的种特异性提供了证据。尽管初步，但这些 VP-1 coproAg ELISA 结果证明有必要通过对更多猪带绦虫和牛带绦虫成虫感染个体的粪便样本进行测试来进一步验证。
