Amino N, Ohse T, Koyano T, Umezawa K
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Anticancer Res. 1996 Jan-Feb;16(1):55-9.
K-ras-NIH3T3 cells were more invasive than NIH3T3 cells in a chemotactic invasion assay. Conophylline, a new vinca alkaloid isolated as a ras function inhibitor, inhibited the invasion of K-ras-NIH3T3 cells, while it showed no effect on NIH3T3 cells. Conophylline did not increase expression of fibronectin but induced E-cadherin expression in K-ras-NIH3T3 cells. Mouse melanoma B16/F10 is a highly metastatic cell line. Conophylline was found to induce flat morphology in B16/F10 cells and it again inhibited the invasion of the cells to the matrigel membrane. It induced fibronectin expression but not E-cadherin expression in B 16/F10 cells. Thus, conophylline lowered invasiveness of K-ras-NIH3T3 and B 16/F10 cells by reversing neoplastic phenotypes.
在趋化侵袭试验中，K-ras-NIH3T3细胞比NIH3T3细胞更具侵袭性。新分离出的作为ras功能抑制剂的长春花生物碱类化合物conophylline抑制了K-ras-NIH3T3细胞的侵袭，而对NIH3T3细胞无作用。Conophylline未增加纤连蛋白的表达，但在K-ras-NIH3T3细胞中诱导了E-钙黏蛋白的表达。小鼠黑色素瘤B16/F10是一种高转移性细胞系。发现conophylline可诱导B16/F10细胞呈现扁平形态，并且再次抑制了细胞向基质胶膜的侵袭。它在B16/F10细胞中诱导了纤连蛋白的表达，但未诱导E-钙黏蛋白的表达。因此，conophylline通过逆转肿瘤表型降低了K-ras-NIH3T3和B16/F10细胞的侵袭性。
