Wun T, Paglieroni T, Lachant N A
Division of Hematology and Oncology, UC Davis School of Medicine, Toledo, Ohio.
Br J Haematol. 1996 Mar;92(4):968-72. doi: 10.1046/j.1365-2141.1996.436975.x.
Arginine vasopressin (AVP) is a neurohypophyseal peptide hormone with protean effects. Previous reports had shown that AVP stimulates platelets, but only at concentrations 3-6 logs higher than the normal plasma concentrations in humans. In this study we tested the hypothesis that AVP, at physiologic concentrations, stimulated the expression of an activation-dependent platelet antigen. Platelets obtained from normal volunteers were incubated with increasing concentrations of AVP and the expression of the activation-dependent platelet antigen P-selectin (CD62) was determined by monoclonal antibodies and flow cytometry. There was a concentration-dependent increase in CD62 expression with increasing AVP concentration; at 1 pm AVP, 24.5% (1.3-88.5%) [median (range)] of platelets expressed CD62. The selective vasopressin V1 receptor antagonist d(CH2)(5)-Tyr(me)AVP (TM-AVP) completely abolished AVP-stimulated CD62 expression. We conclude that AVP can activate platelets at concentrations found in normal humans, at least in vitro, and that this response is mediated by the platelet V1 receptor. AVP may be a physiologic platelet agonist.
精氨酸加压素（AVP）是一种具有多种效应的神经垂体肽类激素。既往报道显示，AVP可刺激血小板，但所需浓度比人类正常血浆浓度高3至6个对数级。在本研究中，我们检验了这样一个假设，即生理浓度的AVP可刺激一种激活依赖性血小板抗原的表达。从正常志愿者获取的血小板与浓度不断增加的AVP一起孵育，通过单克隆抗体和流式细胞术测定激活依赖性血小板抗原P-选择素（CD62）的表达。随着AVP浓度增加，CD62表达呈浓度依赖性增加；在1 pm AVP时，24.5%（1.3 - 88.5%）[中位数（范围）]的血小板表达CD62。选择性加压素V1受体拮抗剂d(CH2)(5)-Tyr(me)AVP（TM-AVP）完全消除了AVP刺激的CD62表达。我们得出结论，至少在体外，AVP在正常人的血浆浓度下即可激活血小板，且这种反应是由血小板V1受体介导的。AVP可能是一种生理性血小板激动剂。
