Baudo M M, Meza-Zepeda L A, Palva E T, Heino P
Department of Molecular Genetics, Uppsala Genetic Center, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Plant Mol Biol. 1996 Jan;30(2):331-6. doi: 10.1007/BF00020118.
A DNA fragment corresponding to a low-temperature- and ABA-responsive gene (Scdhn1) was amplified by PCR from genomic DNA of a wild, frost-resistant potato species, Solanum commersonii. A homologous gene (Stdhn1) was identified in Solanum tuberosum cv. Bintje, a frost-sensitive domesticated potato cultivar. The expression of the gene was studied during low temperature and ABA treatments in both Solanum species. The analysis revealed that both low temperature and ABA lead to the accumulation of a 1 kb transcript that corresponded to the PCR fragment. The induction of the gene was relatively rapid and maximum amounts of the transcripts were detected already after 1 day and 7 h of treatment with low temperature and ABA, respectively. Previous results have shown that there is no increase in the amount of endogenous ABA in S. tuberosum during low-temperature treatment, which indicates that two independent signalling pathways lead to the induction of this gene.
通过聚合酶链反应（PCR）从野生抗霜马铃薯物种——康氏茄（Solanum commersonii）的基因组DNA中扩增出一段与低温和脱落酸（ABA）响应基因（Scdhn1）对应的DNA片段。在对霜敏感的栽培马铃薯品种宾杰（Solanum tuberosum cv. Bintje）中鉴定出了一个同源基因（Stdhn1）。对这两个茄科物种在低温和ABA处理过程中该基因的表达进行了研究。分析表明，低温和ABA均导致了与PCR片段相对应的1 kb转录本的积累。该基因的诱导相对较快，分别在低温和ABA处理1天和7小时后就检测到了最大量的转录本。先前的结果表明，在低温处理期间马铃薯块茎中内源ABA的量没有增加，这表明两条独立的信号通路导致了该基因的诱导。
