Nakamura H, Satoh W, Hidaka S, Kagaya Y, Ejiri S, Tsutsumi K
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Japan.
Plant Mol Biol. 1996 Jan;30(2):381-5. doi: 10.1007/BF00020125.
Complementary and genomic DNA clones coding for aldolase C-1, the fourth-type isozyme of aldolase in rice Oryza sativa L., have been characterized. The organization of the gene is quite similar to those encoding rice aldolase C-a and a maize cytoplasmic-type aldolase, in that introns are located in the same position. Amino acid sequences are highly conserved among cytoplasmic aldolases in plants. Expression of the gene in rice callus is activated by a protein phosphatase inhibitor okadaic acid, and is inhibited in the presence of thapsigargin, a reagent which increases calcium influx into the cytoplasm. The inhibition is rescued by the simultaneous addition of protein kinase inhibitor H-7. Thus, it is suggested that expression of the aldolase C-1 gene is regulated through a signal transduction pathway involving a Ca 2+ -mediated protein kinase-protein phosphatase system.
已对编码水稻（Oryza sativa L.）醛缩酶第四类同工酶醛缩酶C-1的互补DNA和基因组DNA克隆进行了表征。该基因的组织与编码水稻醛缩酶C-a和玉米细胞质型醛缩酶的基因非常相似，因为内含子位于相同位置。植物细胞质醛缩酶之间的氨基酸序列高度保守。水稻愈伤组织中该基因的表达被蛋白磷酸酶抑制剂冈田酸激活，并在毒胡萝卜素（一种增加钙流入细胞质的试剂）存在下受到抑制。同时添加蛋白激酶抑制剂H-7可挽救这种抑制作用。因此，表明醛缩酶C-1基因的表达是通过涉及Ca2+介导的蛋白激酶-蛋白磷酸酶系统的信号转导途径来调节的。
