Ripley T L, Little H J
Pharmacology Department, Medical School, University Walk, Bristol, UK.
Brain Res. 1995 Nov 13;699(1):1-11. doi: 10.1016/0006-8993(95)00445-v.
Hippocampal slices were prepared immediately after withdrawal from chronic ethanol in vivo. The decreases in thresholds for production of single and multiple population spikes seen after the ethanol treatment were not evident when CGP39551 was included in the perfusion medium at 20 microM. The decrease in paired pulse potentiation seen during ethanol withdrawal, however, was not prevented by CGP39551. For comparison, the effects of CGP39551, at the same concentration, were examined on the changes in field potentials seen in control slices when the magnesium concentration in the bathing medium was lowered to 250 microM. The decreases in thresholds for multiple population spikes produced by the lowered magnesium were prevented, but not other changes including decreases in single spike thresholds. In addition, this 20 microM concentration of CGP39551 did not prevent epileptiform activity, measured by decreases in thresholds for production of single and multiple population spikes caused by addition of the GABAA antagonist, bicuculline, to control hippocampal slices.
海马切片在体内从慢性乙醇戒断后立即制备。当在灌注培养基中加入20微摩尔的CGP39551时，乙醇处理后观察到的单一群峰和多群峰产生阈值的降低并不明显。然而，CGP39551并不能阻止乙醇戒断期间配对脉冲增强的降低。为了进行比较，在相同浓度下，研究了CGP39551对对照切片中当浴液中镁浓度降低至250微摩尔时所观察到的场电位变化的影响。降低镁浓度所产生的多群峰阈值的降低被阻止了，但其他变化，包括单峰阈值的降低，并未被阻止。此外，20微摩尔浓度的CGP39551并不能阻止癫痫样活动，癫痫样活动通过向对照海马切片中加入GABAA拮抗剂荷包牡丹碱所导致的单一群峰和多群峰产生阈值的降低来衡量。
