Oliff H S, Weber E, Miyazaki B, Marek P
Department of Pharmacology, College of Medicine, University of California, Irvine 92717, USA.
Brain Res. 1995 Nov 20;699(2):329-31. doi: 10.1016/0006-8993(95)01045-w.
We recently reported that the outcome of focal cerebral ischemia induced by intraluminal middle cerebral artery occlusion may differ depending upon a rat's strain and/or vendor. Sprague-Dawley rats originating from Taconic Laboratories and Charles River Laboratories had infarct volumes several fold larger than Sprague-Dawley rats originating from Simonsen Laboratories. The present study sought to determine whether these differences were restricted to an intraluminal technique or whether strain and vendor dependent differences will also exist in rats subjected to a transcranial method of focal cerebral ischemia. Accordingly, we permanently coagulated the middle cerebral artery via a transcranial approach in Sprague-Dawley rats originating from Simonsen Laboratories and Taconic Laboratories and in Simonsen Laboratories Fischer-344 rats. The cortical infarct volume significantly differed in the following order: Simonsen Laboratories Sprague-Dawley rats < Simonsen Laboratories Fischer-344 rats < Taconic Laboratories Sprague-Dawley rats. The subcortical infarct volume differed statistically in the following order: Simonsen Laboratories Sprague-Dawley rats < Taconic Laboratories Sprague-Dawley rats < Simonsen Laboratories Fischer-344 rats. These results along with our previous findings demonstrate that strain and vendor differences in the outcome of focal cerebral ischemia are independent of the technique applied.
我们最近报道，大脑中动脉腔内闭塞诱导的局灶性脑缺血的结果可能因大鼠的品系和/或供应商而异。来自塔科尼克实验室和查尔斯河实验室的斯普拉格-道利大鼠的梗死体积比来自西蒙森实验室的斯普拉格-道利大鼠大几倍。本研究旨在确定这些差异是否仅限于腔内技术，或者在采用经颅局灶性脑缺血方法的大鼠中是否也存在品系和供应商依赖性差异。因此，我们通过经颅途径对来自西蒙森实验室和塔科尼克实验室的斯普拉格-道利大鼠以及西蒙森实验室的费希尔-344大鼠永久性凝固大脑中动脉。皮质梗死体积按以下顺序有显著差异：西蒙森实验室斯普拉格-道利大鼠＜西蒙森实验室费希尔-344大鼠＜塔科尼克实验室斯普拉格-道利大鼠。皮质下梗死体积按以下顺序有统计学差异：西蒙森实验室斯普拉格-道利大鼠＜塔科尼克实验室斯普拉格-道利大鼠＜西蒙森实验室费希尔-344大鼠。这些结果以及我们之前的发现表明，局灶性脑缺血结果中的品系和供应商差异与所应用的技术无关。
