Hsu C H, Chua K Y, Tao M H, Lai Y L, Wu H D, Huang S K, Hsieh K H
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Nat Med. 1996 May;2(5):540-4. doi: 10.1038/nm0596-540.
The efficacy of an "allergen-gene immunization" protocol in altering allergic response was examined. Intramuscular injection of rats with a plasmid DNA encoding a house dust mite allergen into the muscle results in its long-term expression and the induction of specific immune responses. Significantly, this approach prevents the induction of immunoglobulin E synthesis, histamine release in bronchoalveolar fluids, and airway hyperresponsiveness in rats challenged with aerosolized allergen. Furthermore, this suppression is persistent and can be transferred into naive rats by CD8+ T cells from gene-immunized rats. These findings suggest that allergen-gene immunization is effective in modulating allergic responses, and may provide a novel therapeutic approach for allergic diseases.
研究了“变应原-基因免疫”方案在改变过敏反应方面的疗效。给大鼠肌肉内注射编码屋尘螨变应原的质粒DNA，可使其长期表达并诱导特异性免疫反应。值得注意的是，这种方法可防止在用雾化变应原攻击的大鼠中诱导免疫球蛋白E合成、支气管肺泡液中组胺释放以及气道高反应性。此外，这种抑制作用持续存在，并且可以通过基因免疫大鼠的CD8 + T细胞转移到未接触过变应原的大鼠中。这些发现表明，变应原-基因免疫在调节过敏反应方面是有效的，并且可能为过敏性疾病提供一种新的治疗方法。
