转化生长因子β超家族成员可指导性地促进神经嵴细胞的不同命运。

Alternative neural crest cell fates are instructively promoted by TGFbeta superfamily members.

作者信息

Shah N M, Groves A K, Anderson D J

机构信息

Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, 91125, USA.

出版信息

Cell. 1996 May 3;85(3):331-43. doi: 10.1016/s0092-8674(00)81112-5.

DOI:10.1016/s0092-8674(00)81112-5

PMID:8616889

Abstract

How growth factors influence the fate of multipotent progenitor cells is not well understood. Most hematopoietic growth factors act selectively as survival factors, rather than instructively as lineage determination signals. In the neural crest, neuregulin instructively promotes gliogenesis, but how alternative fates are determined is unclear. We demonstrate that bone morphogenic protein 2 (BMP2) induces the basic-helix-loop-helix protein MASH1 and neurogenesis in neural crest stem cells. In vivo, MASH1+ cells are located near sites of BMP2 mRNA expression. Some smooth muscle differentiation is also observed in BMP2. A related factor, transforming growth factor beta1 (TGFbeta1), exclusively promotes smooth muscle differentiation. Like neuregulin, BMP2 and TGFbeta1 act instructively rather than selectively. The neural crest and hematopoietic systems may therefore utilize growth factors in different ways to generate cellular diversity.

摘要

生长因子如何影响多能祖细胞的命运尚未完全明确。大多数造血生长因子选择性地作为存活因子起作用，而非作为谱系决定信号起指令性作用。在神经嵴中，神经调节蛋白具有指令性地促进神经胶质细胞生成，但尚不清楚其他命运是如何被决定的。我们证明骨形态发生蛋白2（BMP2）可诱导神经嵴干细胞中碱性螺旋-环-螺旋蛋白MASH1的表达及神经发生。在体内，MASH1+细胞位于BMP2 mRNA表达部位附近。在BMP2处理的细胞中也观察到了一些平滑肌分化现象。一种相关因子，转化生长因子β1（TGFβ1），专门促进平滑肌分化。与神经调节蛋白一样，BMP2和TGFβ1起指令性作用而非选择性作用。因此，神经嵴和造血系统可能以不同方式利用生长因子来产生细胞多样性。