Torcia M, Bracci-Laudiero L, Lucibello M, Nencioni L, Labardi D, Rubartelli A, Cozzolino F, Aloe L, Garaci E
Department of Experimental Medicine, University of Rome "Tor Vergata", Italy.
Cell. 1996 May 3;85(3):345-56. doi: 10.1016/s0092-8674(00)81113-7.
Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD "virgin" B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific IgG in mice immunized with tetanus toxoid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.
在人T淋巴细胞、B淋巴细胞和巨噬细胞培养物中评估了神经生长因子（NGF）的产生。只有B细胞组成性地产生NGF，B细胞还表达表面p140trk - A和p75NGFR分子，因此能有效结合并内化该细胞因子。内源性NGF的中和导致Bcl - 2蛋白消失以及带有表面IgG或IgA的静息淋巴细胞凋亡死亡，这一群体包含记忆细胞，而表面IgM/IgD“原始”B淋巴细胞未受影响。在体内给予中和性抗NGF抗体导致用破伤风类毒素、硝基苯酚或砷酸盐免疫的小鼠中特异性IgG滴度大幅降低，且表面IgG或IgA B淋巴细胞数量减少。因此，NGF是记忆B淋巴细胞的自分泌存活因子。
