Macey M G, Howells G L, Stone S R, Newland A C
Department of Haematology, Royal London Hospital, Whitechapel, UK.
Leukemia. 1996 Apr;10(4):687-92.
The platelet-type thrombin receptor was expressed by large granular lymphocytes (LGLs) in a variety of proliferative diseases. Twenty patients with LGL proliferative disease were examined, including five T cell clones and a variety of polyclonal proliferations, some secondary to rheumatoid arthritis and Felty's syndrome; 17/20 showed high number of CD3+, CD8+, and CD57+ lymphocytes and 9/20 also had high numbers of CD16+ or CD 56+ positive lymphocytes. The thrombin receptor was present on more than 20% of the LGLs in 13/20 patients. The clonal T cell expansions showed the highest receptor expression with greater than 75% cells positive. Regression analysis of all 20 cases showed striking and highly statistically significant positive Spearman rank correlation between the proportion of thrombin receptor and CD57-positive LGLs (r = 0.56, P = 0.009). A negative correlation with CD56 was also found (r = -0.46, P= 0.043). Dual antibody flow cytometry showed the receptor was more often co-expressed with CD57 (64%) than with CD16 (19%) or CD56 (11%). The expression of the platelet-type thrombin receptor by LGLs of this phenotype raises the possibility of a functional role for thrombin in the pathogenesis of LGL proliferative diseases.
血小板型凝血酶受体在多种增殖性疾病的大颗粒淋巴细胞（LGL）中表达。对20例LGL增殖性疾病患者进行了检查，包括5个T细胞克隆和多种多克隆增殖，其中一些继发于类风湿关节炎和费尔蒂综合征；20例中有17例显示CD3 +、CD8 +和CD57 +淋巴细胞数量较高，20例中有9例CD16 +或CD56 +阳性淋巴细胞数量也较高。20例患者中有13例LGL上的凝血酶受体存在率超过20%。克隆性T细胞扩增显示受体表达最高，超过75%的细胞呈阳性。对所有20例病例的回归分析显示，凝血酶受体比例与CD57阳性LGL比例之间存在显著且具有高度统计学意义的正Spearman等级相关性（r = 0.56，P = 0.009）。还发现与CD56呈负相关（r = -0.46，P = 0.043）。双抗体流式细胞术显示，该受体与CD57共表达的情况更常见（64%），而与CD16（19%）或CD56（11%）共表达的情况较少。这种表型的LGL表达血小板型凝血酶受体增加了凝血酶在LGL增殖性疾病发病机制中发挥功能作用的可能性。
