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溶液中HLA - DR1.(葡萄球菌肠毒素A)2三聚体的分离。

Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution.

作者信息

Tiedemann R E, Urban R J, Strominger J L, Fraser J D

机构信息

Department of Molecular Medicine, School of Medicine, University of Auckland, New Zealand.

出版信息

Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12156-9. doi: 10.1073/pnas.92.26.12156.

DOI:10.1073/pnas.92.26.12156
PMID:8618863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC40315/
Abstract

Mutational studies indicate that the superantigen staphylococcal enterotoxin A (SEA) has two separate binding sites for major histocompatibility complex (MHC) class II molecules. Direct evidence is provided here for the formation of SEA-MHC class II trimers in solution. Isoelectric focusing separated SEA-HLA-DR1 complexes into both dimers and HLA-DR1.SEA2 trimers. The molar ratio of components was determined by dual isotope labeling. The SEA mutant SEA-F47S, L48S, Y92A, which is deficient in MHC class II alpha-chain binding, formed only dimers with HLA-DR1, whereas a second SEA mutant, SEA-H225A, which lacks high-affinity MHC class II beta-chain binding was incapable of forming any complexes. Thus SEA binding to its MHC receptor is a two-step process involving initial beta-chain binding followed by cooperative binding of a second SEA molecule to the class II alpha chain.

摘要

突变研究表明，超抗原葡萄球菌肠毒素A（SEA）对主要组织相容性复合体（MHC）II类分子有两个独立的结合位点。本文提供了溶液中SEA-MHC II类三聚体形成的直接证据。等电聚焦将SEA-HLA-DR1复合物分离为二聚体和HLA-DR1.SEA2三聚体。通过双同位素标记确定各组分的摩尔比。缺乏MHC II类α链结合能力的SEA突变体SEA-F47S、L48S、Y92A仅与HLA-DR1形成二聚体，而另一个缺乏高亲和力MHC II类β链结合能力的SEA突变体SEA-H225A则无法形成任何复合物。因此，SEA与其MHC受体的结合是一个两步过程，首先是β链结合，随后是第二个SEA分子与II类α链的协同结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/b424a77b2e91/pnas01504-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/04cc190a286e/pnas01504-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/acc7bdc98c1c/pnas01504-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/b424a77b2e91/pnas01504-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/04cc190a286e/pnas01504-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/acc7bdc98c1c/pnas01504-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbda/40315/b424a77b2e91/pnas01504-0221-a.jpg

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