Itoh Y, Ando M, Ohmura M, Kondo A, Miyake K, Saito M
Department of Urology, Kasugia Municipal Hospital, Japan.
Hinyokika Kiyo. 1996 Mar;42(3):215-20.
An adverse effect of the administration of luteinizing hormone-releasing hormone (LH-RH) analogue is impotence. The effects of LH-RH analogue injection on the function of the rabbit corpus cavernosum were investigated. Eighteen male rabbits were divided into three groups, i.e., LH-RH analogue injection, castration, and sham-operated control groups. After measurement of serum testosterone, the LH-RH analogue (1.5 mg/kg leuprolide acetate) was injected once in the LH-RH group, castration in the castrated group, and sham surgery in the control group. Four weeks later, the rabbits were maintained in the same circumstance and serum testosterone was measured once a week. Four weeks after preparation, all rabbits were used for in vitro experiments. At one week the serum testosterone level of the LH-RH and castrated groups decreased significantly from that in the sham-operated control group, which was sustained for the next 3 weeks. Although contractile strength of the corporal tissue taken from the castrated group was weakened in response to phenylephrine and KCl, corporal contractile strength of the LH-RH group was not. Under precontraction with 200 microM phenylephrine relaxation of the corpus cavernosum in response to field stimulation and sodium nitroprusside significantly decreased in both the LH-RH and castrated groups. However, there were no differences in the maximal relaxations induced by ATP and bethanechol between the three groups. In conclusion, the LH-RH analogue impaired the relaxation of the corpus cavernosum induced by field stimulation and sodium nitroprusside as much as castration did. Although the corporal contraction to phenylephrine and KCl was decreased by castration, it was not altered by the LH-RH analogue.
促黄体生成素释放激素（LH-RH）类似物给药的一个不良反应是阳痿。研究了LH-RH类似物注射对兔海绵体功能的影响。18只雄性兔分为三组，即LH-RH类似物注射组、去势组和假手术对照组。测定血清睾酮后，LH-RH组注射一次LH-RH类似物（1.5mg/kg醋酸亮丙瑞林），去势组进行去势，对照组进行假手术。四周后，将兔饲养在相同环境中，每周测定一次血清睾酮。制备四周后，所有兔用于体外实验。在第一周，LH-RH组和去势组的血清睾酮水平与假手术对照组相比显著降低，并在接下来的3周内持续下降。虽然去势组取材的海绵体组织对去氧肾上腺素和氯化钾的收缩强度减弱，但LH-RH组的海绵体收缩强度未减弱。在200μM去氧肾上腺素预收缩下，LH-RH组和去势组海绵体对电场刺激和硝普钠的舒张反应均显著降低。然而，三组之间ATP和氨甲酰甲胆碱诱导的最大舒张无差异。总之，LH-RH类似物对电场刺激和硝普钠诱导的海绵体舒张的损害与去势一样严重。虽然去势会降低海绵体对去氧肾上腺素和氯化钾的收缩，但LH-RH类似物不会改变这种收缩。
