Yamamura T, Hitomi J, Nagasaki K, Suzuki M, Takahashi E, Saito S, Tsukada T, Yamaguchi K
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Apr 16;221(2):356-60. doi: 10.1006/bbrc.1996.0600.
We have isolated and characterized a cDNA and the gene of the human homologue of CAAF1, a novel member of the S100 calcium-binding protein family. The 276-bp open reading frame encoded a 92-amino acid polypeptide with a predicted molecular mass of 10,575 Da. The deduced amino acid sequence of human CAAF1 showed 66% homology to bovine CAAF1. The human CAAF1 gene consisted of three exons, with the two EF hand motifs of the CAAF1 protein separately encoded by exons 2 and 3. This gene was expressed at a high level in polymorphonuclear leukocytes and at an intermediate level in esophageal mucosa. The tissue distribution of CAAF1 mRNA was different from that of other S100 proteins. Direct R-banding fluorescence in situ hybridization revealed that the human CAAF1 gene was mapped to chromosome 1q21.2-q22, where most of the S100 genes form a cluster.
我们已经分离并鉴定了一种cDNA以及CAAF1人类同源物的基因，CAAF1是S100钙结合蛋白家族的一个新成员。这个276 bp的开放阅读框编码了一个92个氨基酸的多肽，预测分子量为10,575 Da。推导的人类CAAF1氨基酸序列与牛CAAF1显示出66%的同源性。人类CAAF1基因由三个外显子组成，CAAF1蛋白的两个EF手基序分别由外显子2和外显子3编码。该基因在多形核白细胞中高水平表达，在食管黏膜中中等水平表达。CAAF1 mRNA的组织分布与其他S100蛋白不同。直接R带荧光原位杂交显示，人类CAAF1基因定位于染色体1q21.2 - q22，大多数S100基因在那里形成一个簇。
