Wicki R, Marenholz I, Mischke D, Schäfer B W, Heizmann C W
Kinderspital, Abteilung für Klinische Chemie, Universität Zürich, Switzerland.
Cell Calcium. 1996 Dec;20(6):459-64. doi: 10.1016/s0143-4160(96)90087-1.
Here, we report the characterization of a human cDNA coding for the recently published amino acid sequence of a calcium-binding S100 protein, S100A12 (CGRP, calgranulin C, CAAF1, p6). The exon/intron structure of the S100A12 gene is similar to most other S100 genes. It is composed of three exons which are divided by two introns of 900 bp and 400 bp. The protein is encoded by sequences in exons 2 and 3, with exon 2 coding for the N-terminal 45 amino acids and exon 3 coding for the C-terminal 46 amino acids. So far, ten S100 genes are known to be located on human chromosome 1q21 in a clustered organization. Hence, we investigated whether S100A11 (S100C, calgizzarin) and S100A12 are also localized in the S100 gene cluster. We found both genes within the cluster, with S100A11 being close to S100A10 and S100A12 between the genes S100A8 and S100A9. Therefore, the S100 gene cluster now is composed of 12 differentially expressed family members.
在此，我们报告了一个人源cDNA的特征，该cDNA编码最近发表的一种钙结合S100蛋白S100A12（降钙素基因相关肽、钙粒蛋白C、CAAF1、p6）的氨基酸序列。S100A12基因的外显子/内含子结构与大多数其他S100基因相似。它由三个外显子组成，被两个分别为900 bp和400 bp的内含子隔开。该蛋白由外显子2和3中的序列编码，外显子2编码N端的45个氨基酸，外显子3编码C端的46个氨基酸。到目前为止，已知有10个S100基因以成簇的形式位于人类染色体1q21上。因此，我们研究了S100A11（S100C、钙磷蛋白）和S100A12是否也定位于S100基因簇中。我们在该基因簇中发现了这两个基因，其中S100A11靠近S100A10，S100A12位于S100A8和S100A9基因之间。因此，S100基因簇现在由12个差异表达的家族成员组成。
