Ohashi K, Sawata M, Takeuchi H, Natori S, Kubo T
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Apr 16;221(2):380-5. doi: 10.1006/bbrc.1996.0604.
Previously, we identified and purified an alpha-glucosidase with a molecular mass of 70 kDa from a homogenate of the hypopharyngeal gland of the older worker bee (forager bee) Apis mellifera L. (T. Kubo et al, J. Biochem., 1996, 119, 291-295). Here, we isolated and sequenced a cDNA for the alpha-glucosidase. The cDNA encoded a protein consisting of 650 amino acids, which had high sequence identity with fruit fly and mosquito possible maltase gene products. RT-PCR showed that the gene was expressed specifically in the hypopharyngeal gland of the forager bee.
此前，我们从意大利蜜蜂（Apis mellifera L.）老年工蜂（采蜜蜂）下咽腺的匀浆中鉴定并纯化了一种分子量为70 kDa的α-葡萄糖苷酶（T. Kubo等人，《生物化学杂志》，1996年，第119卷，第291 - 295页）。在此，我们分离并测定了该α-葡萄糖苷酶的cDNA序列。该cDNA编码一种由650个氨基酸组成的蛋白质，它与果蝇和蚊子可能的麦芽糖酶基因产物具有高度的序列同一性。逆转录聚合酶链反应（RT-PCR）表明该基因在采蜜蜂的下咽腺中特异性表达。
