Inokuchi K, Murayama A, Ozawa F
Mitsubishi Kasei Insitute of Life Sciences, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Apr 16;221(2):430-6. doi: 10.1006/bbrc.1996.0612.
The prolonged maintenance of hippocampal long-term potentiation depends on de novo protein and RNA synthesis, indicating an involvement of altered gene expression in long-lasting plastic changes in synaptic efficacy. We have used an mRNA differential display technique to identify a set of genes that are induced by neural activity in the rat hippocampus. Sixteen independent cDNAs were isolated whose mRNA level was markedly modulated by convulsive seizure. One of these encodes Krox-20, a zinc finger DNA binding protein. High frequency tetanic stimulation of perforant pathway, which elicited a persistent long-term potentiation (>10 h), rapidly induced expression of krox-20 mRNA in the hippocampus of urethane-anesthetized rat. The increase in krox-20 mRNA was transient and NMDA receptor-dependent. These results suggest a role for krox-20 in the maintenance of long-term potentiation.
海马体长期增强效应的持续维持依赖于从头合成蛋白质和RNA，这表明基因表达的改变参与了突触效能的持久可塑性变化。我们使用mRNA差异显示技术来鉴定一组由大鼠海马体中的神经活动诱导的基因。分离出了16个独立的cDNA，其mRNA水平受到惊厥发作的显著调节。其中一个编码Krox-20，一种锌指DNA结合蛋白。对穿通通路进行高频强直刺激，可引发持续的长期增强效应（>10小时），能迅速诱导在乌拉坦麻醉大鼠海马体中krox-20 mRNA的表达。krox-20 mRNA的增加是短暂的且依赖于NMDA受体。这些结果表明krox-20在长期增强效应的维持中发挥作用。
