Isaacs H V, Pownall M E, Slack J M
Department of Zoology, University of Oxford, United Kingdom.
Int J Dev Biol. 1995 Aug;39(4):575-9.
A detailed study of the expression pattern of embryonic fibroblast growth factor (eFGF) during early Xenopus development has been undertaken using whole-mount DIG in situ hybridization. We show that the zygotic expression of eFGF is activated in the mesoderm of the early gastrula and is first visualized as a ring around the blastopore, with significantly higher levels of expression on the dorsal side of the embryo. As gastrulation proceeds, eFGF transcripts become increasingly abundant in the dorsal blastopore lip. In the early neurula eFGF expression can be detected in the extreme posterior of the embryo around the closed blastopore and in the cells of the notochord. This latter result is significant and represents the first report of a Xenopus FGF that is expressed in the notochord. In addition, we show that during gastrula and neurula stages, expression of eFGF closely follows the expression of the Xenopus brachyury (Xbra) gene. During later development eFGF expression is localized to the tail-bud region and a stripe at the mid-brain/hind-brain junction. These data provide further evidence that FGFs play an important role in regulating the expression of brachyury in the developing mesoderm.
利用整装地高辛原位杂交技术，对非洲爪蟾早期发育过程中胚胎成纤维细胞生长因子（eFGF）的表达模式进行了详细研究。我们发现，eFGF的合子表达在早期原肠胚的中胚层中被激活，最初表现为围绕胚孔的一个环，胚胎背侧的表达水平明显更高。随着原肠胚形成的进行，eFGF转录物在背侧胚孔唇中变得越来越丰富。在早期神经胚中，eFGF表达可在胚胎的最后端围绕闭合的胚孔以及脊索细胞中检测到。后一结果意义重大，代表了在非洲爪蟾中首次报道有FGF在脊索中表达。此外，我们还表明，在原肠胚和神经胚阶段，eFGF的表达与非洲爪蟾短尾（Xbra）基因的表达密切相关。在后期发育过程中，eFGF表达定位于尾芽区域以及中脑/后脑交界处的一条带。这些数据进一步证明，FGF在调节发育中的中胚层中短尾基因的表达方面发挥着重要作用。
