Bell C
Scand J Immunol. 1979 Mar;9(3):209-16. doi: 10.1111/j.1365-3083.1979.tb02724.x.
The light (L) and heavy (H) chain and the idiotypic (Id) composition of the antibody (Ab) plaque-forming cells (PFC) and serum Ab specific for alpha-1,3 dextran have been characterized in murine strains exhibiting the CH-Ig-lb to e allotypes and in their F1 hybrids with Ig-la1, BALB/c prototypes. The Ab response of the Ig-lb to e mice to the alpha-1,3 dextran was low in the kappa (kappa) L chain class with only a minor, sporadic Ab in the lambda (lambda) L chain class discernible after prolonged immunization. Two of a total of sixty-eight C57Bl/6 Ig-lb mice, in a total of 318 Ig-lb to e mice tested, exhibited, in late responses, a significantly elevated Ab in the lambda L class at both serum and PFC levels, equalling at the PFC level the total non-specific lambda PFC values. An Id analysis showed this lambda Ab and the kappa Ab to lack the Id relatedness to the three BALB/c alpha-1,3 dextran-binding myeloma proteins (MP) Ab prototypes, J-558, 104 E, and UPC-102, exhibited by the lambda Id+ Ab of the Ig-la1 BALB/c prototypes and their F1 hybrids with the Ig-lb to e prototypes. Furthermore, affinity differences could be detected by alpha-1,3 nigerodextrans, PFC inhibition analysis, between the late C57Bl/6 anti-alpha-1,3 dextran lambda Id--Ab PFC and the lambda Id+ Ab PFC of the BALB/c and their F1 progeny.
已对表现出CH-Ig-lb至e同种异型的小鼠品系及其与Ig-la1 BALB/c原型的F1杂种中，针对α-1,3葡聚糖的抗体（Ab）噬斑形成细胞（PFC）和血清Ab的轻链（L）、重链（H）以及独特型（Id）组成进行了表征。Ig-lb至e小鼠对α-1,3葡聚糖的Ab反应在κ轻链（κ）类别中较低，在延长免疫后，仅在λ轻链（λ）类别中可辨别出少量散发性Ab。在总共318只测试的Ig-lb至e小鼠中，68只C57Bl/6 Ig-lb小鼠中有两只在晚期反应中，血清和PFC水平上λ轻链类别中的Ab显著升高，在PFC水平上等于总的非特异性λPFC值。独特型分析表明，这种λ Ab和κ Ab与三种BALB/cα-1,3葡聚糖结合骨髓瘤蛋白（MP）Ab原型J-558、104 E和UPC-102缺乏独特型相关性，而Ig-la1 BALB/c原型及其与Ig-lb至e原型的F1杂种的λ Id+ Ab表现出这种相关性。此外，通过α-1,3黑曲霉葡聚糖进行PFC抑制分析，可以检测到晚期C57Bl/6抗α-1,3葡聚糖λ Id- -Ab PFC与BALB/c及其F1后代的λ Id+ Ab PFC之间的亲和力差异。
