Chrostek L, Szmitkowski M
Department of Biochemical Diagnostics, Medical School, Białystok, Poland.
Eur J Clin Chem Clin Biochem. 1995 Nov;33(11):825-9. doi: 10.1515/cclm.1995.33.11.825.
We examined the activities of class I and II alcohol dehydrogenase isoenzymes in the sera of patients with viral hepatitis using the fluorogenic substrates 4-methoxy-1-naphthaldehyde for class I and 6-methoxy-2-naphthaldehyde for class II. It was found that serum activities of class I and II alcohol dehydrogenase isoenzymes over the course of five weeks of hospitalisation were higher than those of controls. The greatest increase in activities was found at the onset of disease, exceeding the mean control value by about 30 fold for class I and 4 fold for class II. These activities were lower than that of aminotransferase but higher than those for lactate dehydrogenase, alkaline phosphatase and gamma-glutamyltransferase. Thereafter, the activity of alcohol dehydrogenase isoenzymes gradually decreased, but did not reach the values of the control groups in the last period of the study. Activities of class I and II alcohol dehydrogenase isoenzymes correlated well with those of alanine and aspartate aminotransferase and lactate dehydrogenase in the first weeks of illness. These results clearly demonstrate that especially the activity of class I alcohol dehydrogenase isoenzyme measured by a fluorimetric method can be a useful marker of liver cell damage in the course of viral hepatitis.
我们使用I类荧光底物4-甲氧基-1-萘甲醛和II类荧光底物6-甲氧基-2-萘甲醛检测了病毒性肝炎患者血清中I类和II类乙醇脱氢酶同工酶的活性。结果发现，住院五周期间，I类和II类乙醇脱氢酶同工酶的血清活性高于对照组。活性增加最大的时候是在疾病发作时，I类超过对照组均值约30倍，II类超过4倍。这些活性低于转氨酶，但高于乳酸脱氢酶、碱性磷酸酶和γ-谷氨酰转移酶。此后，乙醇脱氢酶同工酶的活性逐渐下降，但在研究的最后阶段未降至对照组的值。在疾病的最初几周，I类和II类乙醇脱氢酶同工酶的活性与丙氨酸氨基转移酶、天冬氨酸氨基转移酶和乳酸脱氢酶的活性密切相关。这些结果清楚地表明，特别是通过荧光法测定的I类乙醇脱氢酶同工酶的活性，可能是病毒性肝炎病程中肝细胞损伤的一个有用标志物。
