Chrostek L, Szmitkowski M, Panasiuk A, Wierzchowski J
Department of Biochemical Diagnostics, Medical Academy of Białystok, Warsaw.
Rocz Akad Med Bialymst. 1996;41(2):268-76.
We examined the activity of class I and II of alcohol dehydrogenase isoenzymes in the sera of patients with viral hepatitis using fluorogenic substrates, 4-methoxy-1-naphthaldehyde for class I and 6-methoxy-2-naphthaldehyde for class II. It was found that serum activities of class I and II of alcohol dehydrogenase isoenzymes during five weeks of hospitalisation were higher than that of control. The greatest increase in activities was found at the onset of disease, and exceeded the mean control value about 30 times for class I and 4 times for class II. These were lower than the aminotransferase activities but higher than the activity of lactate dehydrogenase, alkaline phosphatase and gamma-glutamyltransferase. In the following periods of investigation the activity of alcohol dehydrogenase isoenzymes gradually decreased, but did not reach the values of the control groups in the last period of the study. Activity of class I and II of alcohol dehydrogenase isoenzymes showed a good correlation with alanine and aspartate aminotransferase and lactate dehydrogenase in the first weeks of the illness. These results clearly demonstrate that particularly the activity of class I of alcohol dehydrogenase isoenzymes measured by a fluorimetric method can be a useful marker of liver cell damage in the course of viral hepatitis.
我们使用荧光底物检测了病毒性肝炎患者血清中Ⅰ类和Ⅱ类乙醇脱氢酶同工酶的活性，Ⅰ类用4-甲氧基-1-萘甲醛，Ⅱ类用6-甲氧基-2-萘甲醛。结果发现，住院五周期间乙醇脱氢酶同工酶Ⅰ类和Ⅱ类的血清活性高于对照组。活性增加最大的时候出现在疾病发作时，Ⅰ类超过对照组平均值约30倍，Ⅱ类超过4倍。这些活性低于转氨酶活性，但高于乳酸脱氢酶、碱性磷酸酶和γ-谷氨酰转移酶的活性。在接下来的研究阶段，乙醇脱氢酶同工酶的活性逐渐下降，但在研究的最后阶段未达到对照组的值。在疾病的最初几周，乙醇脱氢酶同工酶Ⅰ类和Ⅱ类的活性与丙氨酸氨基转移酶、天冬氨酸氨基转移酶和乳酸脱氢酶显示出良好的相关性。这些结果清楚地表明，特别是通过荧光法测定的乙醇脱氢酶同工酶Ⅰ类的活性，可能是病毒性肝炎病程中肝细胞损伤的一个有用标志物。
