Aberg F, Zhang Y, Teclebrhan H, Appelkvist E L, Dallner G
Department of Biochemistry, Stockholm University, Sweden.
Chem Biol Interact. 1996 Jan 5;99(1-3):205-18. doi: 10.1016/0009-2797(95)03670-9.
Rats were treated with various peroxisome proliferators and concomitant changes in ubiquinone levels were monitored. In addition to clofibrate and di(2-ethylhexyl)phthalate, acetylsalicylic acid, 2-ethylhexanoic acid, thyroxine and dehydroepiandrosterone were used as proliferators. Administration of these compounds increased the contents of ubiquinone in liver and, to some extent, in kidney and muscle. No change in corresponding valued for heart or brain were observed. The treatments did not influence cholesterol levels, but increased the amounts of dolichol in the liver to various extents. Treatment of rats with the catalase inhibitor aminotriazole increased the ubiquinone levels in kidney, heart and muscle but not in liver. Comparison of peroxisomal fatty acid beta-oxidation with ubiquinone amounts in liver homogenates after treatment with a number of peroxisome proliferators demonstrated a direct correlation between these two parameters. Subcellular fractionation of liver after peroxisome proliferation revealed that the ubiquinone level was increased in mitochondria and lysosomes which are the main compartments for this lipid, but an increase was also observed in both peroxisomes and microsomes. The increase in hepatic ubiquinone after treatment with various types of proliferators was related to the decrease in blood cholesterol level. These results show that the volume of the peroxisomal compartment and the ubiquinone content in animal tissues are interrelated.
用各种过氧化物酶体增殖剂处理大鼠，并监测辅酶Q水平的相应变化。除了氯贝丁酯和邻苯二甲酸二（2-乙基己基）酯外，还使用乙酰水杨酸、2-乙基己酸、甲状腺素和脱氢表雄酮作为增殖剂。给予这些化合物会增加肝脏中辅酶Q的含量，在一定程度上也会增加肾脏和肌肉中的含量。未观察到心脏或大脑的相应值有变化。这些处理不影响胆固醇水平，但会不同程度地增加肝脏中多萜醇的含量。用过氧化氢酶抑制剂氨基三唑处理大鼠会增加肾脏、心脏和肌肉中辅酶Q的水平，但不会增加肝脏中的水平。在用多种过氧化物酶体增殖剂处理后，比较肝脏匀浆中过氧化物酶体脂肪酸β氧化与辅酶Q含量，结果表明这两个参数之间存在直接相关性。过氧化物酶体增殖后肝脏的亚细胞分级分离显示，线粒体和溶酶体（这种脂质的主要存在部位）中的辅酶Q水平升高，但在过氧化物酶体和微粒体中也观察到了增加。用各种类型的增殖剂处理后肝脏中辅酶Q的增加与血液胆固醇水平的降低有关。这些结果表明，动物组织中过氧化物酶体区室的大小与辅酶Q含量是相互关联的。
