Inoue T, Osada H, Shiigai T, Fujiseki M, Shimono M
Department of Pathology, Tokyo Dental College, Japan.
Bull Tokyo Dent Coll. 1993 Nov;34(4):183-90.
Osteogenic activity of autografted dental pulp, periodontal ligament, and bone marrow of rat in vivo was investigated. Immunolocalization of ALPase in situ was also studied. One month after the transplantation, osteodentin was formed in all the dental pulp transplants (100%), bone or cementum like tissues were created in 20% of periodontal ligament transplants, and bone like tissues were in 20% of bone marrow transplants. After two months, osteodentin was produced in all the dental pulp transplants (100%) and bone like tissue were in 50% of both periodontal ligament and bone marrow transplants. Immunohistochemically, positive reactions to ALPase in situ were detected in cells just below the odontoblast layers in dental pulp, surface layers of alveolar bone in periodontal ligament, and endosteal membrane of bone marrow space. From these results, it was suggested that the cells of these three kinds of tissue can be termed osteogenic-fibroblasts in vivo.
研究了大鼠自体移植牙髓、牙周膜和骨髓在体内的成骨活性。还研究了碱性磷酸酶的原位免疫定位。移植后1个月，所有牙髓移植（100%）均形成骨样牙本质，20%的牙周膜移植形成骨或牙骨质样组织，20%的骨髓移植形成骨样组织。2个月后，所有牙髓移植（100%）均产生骨样牙本质，牙周膜和骨髓移植的50%形成骨样组织。免疫组织化学显示，在牙髓成牙本质细胞层下方的细胞、牙周膜牙槽骨表层以及骨髓腔内的骨内膜中均检测到碱性磷酸酶的原位阳性反应。从这些结果推测，这三种组织的细胞在体内可称为成骨成纤维细胞。
