Hirukawa T
The First Department of Prosthetic Dentistry, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Aichi Gakuin Daigaku Shigakkai Shi. 1990 Mar;28(1 Pt 2):367-87.
The purpose of the present study was to investigate the histological changes of the periodontal tissues in rats produced by oral administration of dihydrotachysterol (DHT). Forty-five rats, 6 weeks old, were divided equally into 9 groups (Groups A-I). Groups A, D and G, control groups, received an administration of corn oil (0.5 ml) once a day for 1, 2 and 4 weeks, respectively. Groups B, E and H received an administration of DHT (25 micrograms) once a day for 1, 2 and 4 weeks, respectively. Groups C, F and I had an administration of DHT (50 micrograms) once a day for 1, 2 and 4 weeks, respectively. The animals of all groups were killed 24 hours after the last administration. The periodontal tissues of the maxillary first molar of all animals were examined histologically and histometrically. Histological and histometrical findings: 1. In Groups A, D and G, control groups, the periodontal ligament, alveolar bone and cementum appeared normal during the entire experimental period. 2. In Group B, the peridontal tissues were almost similar to those of the control group. 3. In Groups C and E, osteoid tissue was observed in the alveolar bone surface. The thickness of the periodontal ligament decreased and the bone marrow spaces were slightly enlarged. The volmetric density of the alveolar bone decreased compared with that of the control groups. 4. In Groups F, H and I, the thickness of the periodontal ligament decreased significantly. In the periodontal ligament, destruction of collagen fibers was observed, and fibroblasts, collagen fibers and oxytalan fibers decreased in number. Dental ankylosis was also found. An abnormal darkly-stained layes (using the PAS staining method) was observed in the cementum surface. The alveolar bone marrow spaces were enlarged and replaced by fibrous tissue. In the histometrical findings, the thickness of the periodontal ligament and the volumetric density of the alveolar bone had decreased, and the volume of the cementum had increased significantly. The result of the present study suggested that oral administration of dihydrotachysterol to the rats induced the changes of the periodontal tissues similar to those seen in aging humans.
本研究的目的是调查口服二氢速甾醇（DHT）对大鼠牙周组织的组织学变化。将45只6周龄的大鼠平均分为9组（A - I组）。A、D和G组为对照组，分别在1、2和4周内每天给予一次玉米油（0.5毫升）。B、E和H组分别在1、2和4周内每天给予一次DHT（25微克）。C、F和I组分别在1、2和4周内每天给予一次DHT（50微克）。所有组的动物在最后一次给药后24小时处死。对所有动物上颌第一磨牙的牙周组织进行组织学和组织计量学检查。组织学和组织计量学结果：1. A、D和G组对照组在整个实验期间牙周韧带、牙槽骨和牙骨质均正常。2. B组牙周组织与对照组几乎相似。3. C组和E组在牙槽骨表面观察到类骨质组织。牙周韧带厚度减小，骨髓腔略有扩大。与对照组相比，牙槽骨的体积密度降低。4. F组、H组和I组牙周韧带厚度显著减小。在牙周韧带中观察到胶原纤维破坏，成纤维细胞、胶原纤维和氧化弹力纤维数量减少。还发现了牙粘连。在牙骨质表面观察到异常深色染色层（使用PAS染色法）。牙槽骨髓腔扩大并被纤维组织取代。在组织计量学结果中，牙周韧带厚度和牙槽骨体积密度降低，牙骨质体积显著增加。本研究结果表明，给大鼠口服二氢速甾醇可诱导牙周组织发生类似于老年人的变化。
