用于大肠杆菌质粒改组的微型F质粒载体的构建
Construction of mini-F plasmid vectors for plasmid shuffling in Escherichia coli.
作者信息
Kato J, Ikeda H
机构信息
Department of Molecular Biology, University of Tokyo, Japan.
出版信息
Gene. 1996 Apr 17;170(1):141-2. doi: 10.1016/0378-1119(95)00865-9.
PMID:8621075
Abstract
We have constructed two Escherichia coli mini-F plasmid vectors, pJK286 and pJK289, which have unique EcoRI, BamHI and HindIII sites downstream from the lac promoter. The mini-F vectors are useful for plasmid shuffling, with which we can efficiently carry out localized mutagenesis.
摘要
我们构建了两种大肠杆菌微型F质粒载体，pJK286和pJK289，它们在乳糖启动子下游具有独特的EcoRI、BamHI和HindIII位点。这些微型F载体可用于质粒洗牌，通过该方法我们能够高效地进行定位诱变。
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