必需基因mviN参与大肠杆菌中胞壁质的合成。
Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli.
作者信息
Inoue Azusa, Murata Yoshimitsu, Takahashi Hiroshi, Tsuji Naoko, Fujisaki Shingo, Kato Jun-ichi
机构信息
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
出版信息
J Bacteriol. 2008 Nov;190(21):7298-301. doi: 10.1128/JB.00551-08. Epub 2008 Aug 15.
Abstract
We isolated a temperature-sensitive mutant with a mutation in mviN, an essential gene in Escherichia coli. At the nonpermissive temperature, mviN mutant cells swelled and burst. An intermediate in murein synthesis, polyprenyl diphosphate-N-acetylmuramic acid-(pentapeptide)-N-acetyl-glucosamine, accumulated in mutant cells. These results indicated that MviN is involved in murein synthesis.
摘要
我们分离出了一个mviN基因发生突变的温度敏感型突变体，mviN是大肠杆菌中的一个必需基因。在非允许温度下，mviN突变体细胞肿胀并破裂。肽聚糖合成的一个中间体，聚异戊二烯二磷酸-N-乙酰胞壁酸-（五肽）-N-乙酰葡糖胺，在突变体细胞中积累。这些结果表明MviN参与肽聚糖的合成。
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本文引用的文献
1
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Mol Microbiol. 2007 Sep;65(6):1582-94. doi: 10.1111/j.1365-2958.2007.05896.x. Epub 2007 Aug 21.
2
Construction of consecutive deletions of the Escherichia coli chromosome.大肠杆菌染色体连续缺失的构建
Mol Syst Biol. 2007;3:132. doi: 10.1038/msb4100174. Epub 2007 Aug 14.
3
Transmembrane transport of peptidoglycan precursors across model and bacterial membranes.肽聚糖前体跨模型膜和细菌膜的跨膜运输。
Mol Microbiol. 2007 May;64(4):1105-14. doi: 10.1111/j.1365-2958.2007.05722.x.
4
The MATE proteins as fundamental transporters of metabolic and xenobiotic organic cations.MATE蛋白作为代谢性和外源性有机阳离子的基本转运体。
Trends Pharmacol Sci. 2006 Nov;27(11):587-93. doi: 10.1016/j.tips.2006.09.001. Epub 2006 Sep 25.
5
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Anal Biochem. 2005 Oct 15;345(2):336-9. doi: 10.1016/j.ab.2005.07.002.
6
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FEMS Microbiol Lett. 2005 Sep 15;250(2):195-200. doi: 10.1016/j.femsle.2005.07.005.
7
Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome.基因组缩减的工程化大肠杆菌细胞的细胞大小和类核组织
Mol Microbiol. 2005 Jan;55(1):137-49. doi: 10.1111/j.1365-2958.2004.04386.x.
8
Evidence that the wzxE gene of Escherichia coli K-12 encodes a protein involved in the transbilayer movement of a trisaccharide-lipid intermediate in the assembly of enterobacterial common antigen.大肠杆菌K-12的wzxE基因编码一种参与肠杆菌共同抗原组装过程中三糖脂质中间体跨膜运动的蛋白质的证据。
J Biol Chem. 2003 May 9;278(19):16534-42. doi: 10.1074/jbc.M301750200. Epub 2003 Mar 5.
9
Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein.脂质连接寡糖跨内质网膜的转运需要Rft1蛋白。
Nature. 2002 Jan 24;415(6870):447-50. doi: 10.1038/415447a.
10
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