来自球形红杆菌的鞭毛基因与鼠伤寒沙门氏菌fliF操纵子的基因以及III型分泌系统的基因同源。
Flagellar genes from Rhodobacter sphaeroides are homologous to genes of the fliF operon of Salmonella typhimurium and to the type-III secretion system.
作者信息
Ballado T, Campos A, Camarena L, Dreyfus G
机构信息
Departamento de Bioenergética, Universidad Nacional Autónoma de México, D.F., México.
出版信息
Gene. 1996 Apr 17;170(1):69-72. doi: 10.1016/0378-1119(95)00855-1.
A flagellar region of the genome of Rhodobacter sphaeroides was cloned and sequenced. Three ORFs were identified and arranged in the same order as fliH, fliI and fliJ of Salmonella typhimurium (St). ORF2 is highly similar to FliI from St (49% similarity) showing Walker's A and B motifs. Similar scores were found with proteins of the type-III secretion system of virulence factors. ORF3 shows 16.4 and 11.1% similarity to FliJ from St and Bacillus subtilis, respectively. This work also shows that ORF3 is similar to HrpJ5 from Pseudomonas syringae (19.2% similarity). It was found that ORF2 and ORF3 start immediately downstream from the adjacent coding region, suggesting a single transcriptional unit.
对球形红细菌基因组的鞭毛区域进行了克隆和测序。鉴定出三个开放阅读框（ORF），其排列顺序与鼠伤寒沙门氏菌（St）的fliH、fliI和fliJ相同。ORF2与St的FliI高度相似（相似度为49%），具有沃克A和B基序。在毒力因子的III型分泌系统蛋白中也发现了类似的得分。ORF3与St和枯草芽孢杆菌的FliJ的相似度分别为16.4%和11.1%。这项研究还表明，ORF3与丁香假单胞菌的HrpJ5相似（相似度为19.2%）。发现ORF2和ORF3紧邻相邻编码区域的下游起始，表明这是一个单一的转录单元。
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