García N, Campos A, Osorio A, Poggio S, González-Pedrajo B, Camarena L, Dreyfus G
Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, UNAM, México 04510 D.F., Mexico.
J Bacteriol. 1998 Aug;180(15):3978-82. doi: 10.1128/JB.180.15.3978-3982.1998.
In this work, the genes that encode the FliM and FliN proteins of Rhodobacter sphaeroides were characterized. These genes are part of a large flagellar gene cluster in which six additional open reading frames encoding products homologous to FliL, FliO, FliP, FliQ, FliR, and FlhB proteins from other bacteria were identified. The inactivation of the fliM gene gave a nonflagellate phenotype (Fla-), suggesting that FliM is required for flagellar assembly. Complementation analysis of this fliM mutant indicated that fliM and fliN transcription starts beyond the 5' end of fliK and terminates after fliN.
在本研究中，对球形红细菌中编码FliM和FliN蛋白的基因进行了表征。这些基因是一个大型鞭毛基因簇的一部分，在该基因簇中，还鉴定出了另外六个开放阅读框，其编码的产物与其他细菌的FliL、FliO、FliP、FliQ、FliR和FlhB蛋白同源。fliM基因的失活产生了无鞭毛表型（Fla-），这表明FliM是鞭毛组装所必需的。对该fliM突变体的互补分析表明，fliM和fliN转录起始于fliK的5'端之外，并在fliN之后终止。
