邮票的聚合酶链式反应（PCR）DNA分型：DNA提取的评估

PCR DNA typing of stamps: evaluation of the DNA extraction.

作者信息

Fridez F, Coquoz R

机构信息

Institut de Police Scientifique et de Criminologie, University of Lausanne, Switzerland.

出版信息

Forensic Sci Int. 1996 Apr 2;78(2):103-10. doi: 10.1016/0379-0738(95)01872-7.

DOI:10.1016/0379-0738(95)01872-7

PMID:8621117

Abstract

Today, the PCR analysis of DNA from the saliva deposited on a stamp of an anonymous letter can lead to an identification. However, this analysis still involves problems, and DNA extraction can be particularly difficult. A comparative study of two DNA extraction methods with two categories of postage stamps was carried out and a purification process was tested. This study shows that the extraction with phenol/chloroform gives much better results than Chelex extraction. A purification process such as the use of Centricon 100 microconcentrators is recommended when an inhibition of PCR is present. This operation, however, results in a large loss of material.

摘要

如今，对匿名信件邮票上唾液中的DNA进行PCR分析可实现身份识别。然而，这种分析仍存在问题，DNA提取可能尤其困难。对两类邮票采用两种DNA提取方法进行了对比研究，并测试了一种纯化过程。该研究表明，苯酚/氯仿提取法的效果比Chelex提取法好得多。当存在PCR抑制时，建议使用如Centricon 100微量浓缩器这样的纯化过程。然而，此操作会导致大量材料损失。