García-Plata D, Lessana-Leibowitch M, Palangie A, Gulliemette J, Sedel D, Méndez L, Mozos E
Department of Medicine, University of Córdoba, Spain.
Invasion Metastasis. 1995;15(3-4):125-34.
The purpose of this study was to investigate the relationship between changes in density and distribution of dendritic cells, both in epidermis and in peritumoral infiltrate, and lymphocyte subset variations in malignant melanomas (MM) of patients belonging to different risk groups. The collective immunoreactive expression of six markers (S100 protein, CD1-a, HLA-DR, CD4, CD8 and CD25) was analyzed in 13 cutaneous malignant melanomas. Changes were observed in density and distribution of Langerhans cells (LC) (S100+, CD1-a+) in the epidermis overlying the tumor, as well as in peritumoral and intratumoral locations, independently of the tumor-invasion level. A decrease was recorded in LC (S100+, CD1-a+) in the epidermis overlying six tumors, whereas most of the MM studied showed an increase of LC (S100+, CD1-a+) in peritumoral infiltrate. The expression of HLA-DR in tumor cells was controversial; it was observed in three moderate-risk MM, but it was negative in high-risk tumors. The percentage of CD4+ cells was in most cases greater than that of CD8+ in the peritumoral infiltrate, irrespective of the degree of histopathological malignancy. The concomitant expression of the lymphocytic activation marker CD25 (receptor for interleukin 2) in lymphocytic infiltrate was variable. Peritumoral infiltrate in three high risk MM contained few CD25+ cells, and a concomitant decrease was recorded in LC. This preliminary report shows that alterations in the density and distribution of LC may be responsible for determining the degree or T lymphocyte activation, and this may be critical for the development of effective tumor-directed immunity. Further studies are required to demonstrate these hypothetical interrelations.
本研究的目的是调查不同风险组患者恶性黑色素瘤（MM）中，表皮和瘤周浸润中树突状细胞密度和分布的变化与淋巴细胞亚群变化之间的关系。对13例皮肤恶性黑色素瘤患者分析了六种标志物（S100蛋白、CD1-a、HLA-DR、CD4、CD8和CD25）的集体免疫反应性表达。观察到肿瘤上方表皮以及瘤周和瘤内部位的朗格汉斯细胞（LC）（S100+、CD1-a+）密度和分布发生变化，与肿瘤浸润水平无关。在覆盖六个肿瘤的表皮中，LC（S100+、CD1-a+）数量减少，而大多数研究的MM在瘤周浸润中LC（S100+、CD1-a+）数量增加。肿瘤细胞中HLA-DR的表达存在争议；在三个中度风险的MM中观察到表达，但在高风险肿瘤中为阴性。无论组织病理学恶性程度如何，瘤周浸润中CD4+细胞的百分比在大多数情况下都高于CD8+细胞。淋巴细胞浸润中淋巴细胞活化标志物CD25（白细胞介素2受体）的伴随表达是可变的。三个高风险MM的瘤周浸润中CD25+细胞很少，同时LC数量减少。这份初步报告表明，LC密度和分布的改变可能是决定T淋巴细胞活化程度的原因，这可能对有效的肿瘤导向免疫的发展至关重要。需要进一步研究来证实这些假设的相互关系。
