Toriyama K, Wen D R, Paul E, Cochran A J
Department of Pathology, School of Medicine, University of California, Los Angeles 90024.
J Invest Dermatol. 1993 Mar;100(3):269S-273S. doi: 10.1111/1523-1747.ep12470135.
We examined the frequency, distribution, and immunophenotype (S-100 protein, CD1) of epidermal Langerhans cells (LC) in the epidermis overlying primary melanoma, and dermal dendritic cells (DC) in the dermis deep to melanoma, and in adjacent normal skin. There is a substantial reduction in S-100+ LC and a lesser decline in CD1+ LC in the epidermis over melanoma. There is a simultaneous increase in the frequency of cells expressing these phenotypes in the dermis deep to tumor. Double-staining studies in progress show coexpression of S-100 and CD1 in most of these cells. The depletion of S-100+/CD1+ DC from the peritumoral epidermis is maximum in deeper (Clark level III-V) and thicker (> 0.76 mm) tumors. The reduction in total DC in peritumoral epidermis is, however, proportionally less than the reduction in S-100+/CD1+ DC owing to an increase in cells that are S-100-/CD1+. The proportion of S-100+/CD1+ dermal DC deep to tumor is similar to that in normal epidermis and dermis, suggesting that there is no increased migration of S-100-/CD1+ DC from tumor-associated epidermis to subjacent dermis. Non-dendritic leukocytes in the dermis deep to tumor were increased in frequency, maximally in the dermis deep to thinner and more superficial melanomas. Most such cells were T lymphocytes with helper/inducer cells predominating. The data presented show substantial alterations in the frequency, distribution, and phenotype of LC/DC as melanoma evolves, alterations that may be critical for development of effective tumor-directed immunity.
我们检测了原发性黑色素瘤上方表皮中表皮朗格汉斯细胞（LC）以及黑色素瘤深部真皮和相邻正常皮肤中真皮树突状细胞（DC）的频率、分布和免疫表型（S-100蛋白、CD1）。黑色素瘤上方表皮中S-100+LC显著减少，CD1+LC减少程度较小。肿瘤深部真皮中表达这些表型的细胞频率同时增加。正在进行的双重染色研究显示，这些细胞中的大多数同时表达S-100和CD1。在较深（克拉克分级III-V级）和较厚（>0.76mm）的肿瘤中，肿瘤周围表皮中S-100+/CD1+DC的耗竭最为明显。然而，由于S-100-/CD1+细胞增加，肿瘤周围表皮中总DC的减少与S-100+/CD1+DC的减少相比成比例较小。肿瘤深部S-100+/CD1+真皮DC的比例与正常表皮和真皮中的比例相似，这表明不存在S-100-/CD1+DC从肿瘤相关表皮向下方真皮的迁移增加。肿瘤深部真皮中的非树突状白细胞频率增加，在较薄和较浅表黑色素瘤深部的真皮中增加最为明显。大多数此类细胞是辅助/诱导细胞占主导的T淋巴细胞。所呈现的数据表明，随着黑色素瘤的发展，LC/DC的频率、分布和表型发生了显著改变，这些改变可能对有效的肿瘤定向免疫的发展至关重要。
