Gordon C, McGurk G, Wallace M, Hastie N D
Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, United Kingdom.
J Biol Chem. 1996 Mar 8;271(10):5704-11. doi: 10.1074/jbc.271.10.5704.
We have isolated a conditional lethal mutant mts3 in the fission yeast Schizosaccharomyces pombe which at the permissive temperature is resistant to the mitotic poison MBC and at the restrictive temperature is defective in metaphase to anaphase transition. The predicted amino acid sequence of mts3+ is 36% identical with the budding yeast gene NIN1. NIN1 cloned into a fission yeast expression vector can rescue both mts3 temperature-sensitive and null alleles demonstrating that NIN1 is the budding yeast homologue of the fission yeast mts3+ gene. The phenotype of the mts3 null is identical with the mts3 ts mutant demonstrating that the phenotype of the mts3 ts mutant is due to loss of mts3+ function. The deduced amino acid sequences of both mts3+ and NIN1 show homology to peptide sequences obtained from subunit 14 of the 26 S protease purified from bovine or human cells.
我们在裂殖酵母粟酒裂殖酵母中分离出了一个条件致死突变体mts3，该突变体在允许温度下对有丝分裂毒物MBC具有抗性，而在限制温度下，中期到后期的转换存在缺陷。mts3+的预测氨基酸序列与芽殖酵母基因NIN1有36%的同一性。克隆到裂殖酵母表达载体中的NIN1可以挽救mts3温度敏感型和无效等位基因，这表明NIN1是裂殖酵母mts3+基因的芽殖酵母同源物。mts3无效突变体的表型与mts3温度敏感型突变体相同，这表明mts3温度敏感型突变体的表型是由于mts3+功能丧失所致。mts3+和NIN1的推导氨基酸序列与从牛或人细胞中纯化的26S蛋白酶亚基14获得的肽序列具有同源性。
