Takahara N, Kashiwagi A, Maegawa H, Shigeta Y
Third Department of Medicine, Shiga University of Medical Science, Japan.
Metabolism. 1996 May;45(5):559-64. doi: 10.1016/s0026-0495(96)90024-4.
Lysophosphatidylcholine (LPC increased monocyte chemoattractant protein-1 (MCP-1) messenger RNA concentrations in human umbilical vein endothelial cells (HUVECs). A time-course study showed that the increase in MCP-1 mRNA levels peaked at 6 hours after treatment with LPC. The effect of LPC on the accumulation of MCP-I mRNA levels in HUVECs depended on LPC concentration, and the maximal effect was obtained at 50 micromol / L LPC, which induced a sixfold increase in MCP-1 mRNA levels. The amount of MCP-1 released from HUVECs measured using an enzyme-linked immunosorbent assay (ELISA) showed a 38% increase in the presence of 50 micromol/L LPC, but not in the presence of phosphatidylcholine or lysophosphatidylethanolamine. Coincubation with staurosporine, a potent inhibitor of protein kinase C (PKC) activity, attenuated the LPC-induced increase in MCP-1 mRNA levels by 53%. These results indicate that LPC can induce an increase in MCP-1 mRNA concentrations and stimulate the release of MCP-1 protein from HUVECs, and that the effect of LPC on the MCP-1 gene may be mediated through activation of the PKC pathway.
溶血磷脂酰胆碱（LPC）可增加人脐静脉内皮细胞（HUVECs）中单核细胞趋化蛋白-1（MCP-1）信使核糖核酸的浓度。一项时间进程研究表明，在用LPC处理6小时后，MCP-1信使核糖核酸水平的增加达到峰值。LPC对HUVECs中MCP-1信使核糖核酸水平积累的影响取决于LPC的浓度，在50微摩尔/升LPC时可获得最大效应，此时MCP-1信使核糖核酸水平增加了六倍。使用酶联免疫吸附测定（ELISA）测量HUVECs释放的MCP-1量，结果显示在50微摩尔/升LPC存在时增加了38%，而在磷脂酰胆碱或溶血磷脂酰乙醇胺存在时则没有增加。与蛋白激酶C（PKC）活性的强效抑制剂星形孢菌素共同孵育，可使LPC诱导的MCP-1信使核糖核酸水平增加减弱53%。这些结果表明，LPC可诱导MCP-1信使核糖核酸浓度增加，并刺激HUVECs释放MCP-1蛋白，且LPC对MCP-1基因的作用可能是通过PKC途径的激活介导的。
