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A single-stranded DNA-binding protein shared by telomeric repeats, the variant surface glycoprotein transcription promoter and the procyclin transcription terminator of Trypanosoma brucei.一种由布氏锥虫的端粒重复序列、可变表面糖蛋白转录启动子和前环素转录终止子共享的单链DNA结合蛋白。
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9
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10
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本文引用的文献

1
RNA polymerase I-mediated protein-coding gene expression in Trypanosoma brucei.布鲁氏锥虫中RNA聚合酶I介导的蛋白质编码基因表达
Parasitol Today. 1992 Dec;8(12):414-8. doi: 10.1016/0169-4758(92)90194-7.
2
The procyclin coat of African trypanosomes (or the not-so-naked trypanosome).非洲锥虫的前环素被膜（或并非那么“裸露”的锥虫）。
Parasitol Today. 1990 Mar;6(3):79-82. doi: 10.1016/0169-4758(90)90216-q.
3
Control of polyadenylation and alternative splicing of transcripts from adjacent genes in a procyclin expression site: a dual role for polypyrimidine tracts in trypanosomes?前环素表达位点相邻基因转录本的聚腺苷酸化和可变剪接的调控：锥虫中嘧啶富集区的双重作用？
Nucleic Acids Res. 1994 Apr 25;22(8):1359-64. doi: 10.1093/nar/22.8.1359.
4
Identification of a new expression site-associated gene in the complete 30.5 kb sequence from the AnTat 1.3A variant surface protein gene expression site of Trypanosoma brucei.
Mol Biochem Parasitol. 1993 Nov;62(1):135-7. doi: 10.1016/0166-6851(93)90189-5.
5
The mechanism of transcription termination by RNA polymerase I.RNA聚合酶I转录终止的机制。
Mol Microbiol. 1994 Apr;12(1):11-5. doi: 10.1111/j.1365-2958.1994.tb00989.x.
6
A model for transcription termination by RNA polymerase I.RNA聚合酶I介导的转录终止模型。
Cell. 1994 Nov 4;79(3):527-34. doi: 10.1016/0092-8674(94)90261-5.
7
Genetic controls for the expression of surface antigens in African trypanosomes.非洲锥虫表面抗原表达的遗传控制
Annu Rev Microbiol. 1994;48:25-52. doi: 10.1146/annurev.mi.48.100194.000325.
8
The 3'-terminal region of the mRNAs for VSG and procyclin can confer stage specificity to gene expression in Trypanosoma brucei.VSG和前环素mRNA的3'末端区域可赋予布氏锥虫基因表达阶段特异性。
EMBO J. 1995 Jun 15;14(12):2925-34. doi: 10.1002/j.1460-2075.1995.tb07292.x.
9
Inducible gene expression in trypanosomes mediated by a prokaryotic repressor.
Science. 1995 May 26;268(5214):1179-83. doi: 10.1126/science.7761835.
10
Trypanosoma brucei: a preferential splicing at the inverted polyadenylation site of the VSG mRNA provides further evidence for coupling between trans-splicing and polyadenylation.布氏锥虫：VSG mRNA 反向聚腺苷酸化位点的优先剪接为反式剪接与聚腺苷酸化之间的偶联提供了进一步证据。
Exp Parasitol. 1995 May;80(3):563-7. doi: 10.1006/expr.1995.1069.

布氏锥虫前环素PARP A单位转录终止子的特性分析

Characterization of a transcription terminator of the procyclin PARP A unit of Trypanosoma brucei.

作者信息

Berberof M, Pays A, Lips S, Tebabi P, Pays E

机构信息

Department of Molecular Biology, University of Brussels, Belgium.

出版信息

Mol Cell Biol. 1996 Mar;16(3):914-24. doi: 10.1128/MCB.16.3.914.

DOI:10.1128/MCB.16.3.914
PMID:8622694
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC231073/
Abstract

The polycistronic procylcin PARP (for procyclic acidic repetitive protein) A transcription unit of Trypanosoma brucei was completely characterized by the mapping of the termination region. In addition to the tandem of procyclin genes and GRESAG 2.1, this 7.5- to 9.5-kb unit contained another gene for a putative surface protein, termed PAG (for procyclin-associated gene) 3. The terminal 3-kb sequence did not contain significant open reading frames and cross-hybridized with the beginning of one or several transcription units specific to the bloodstream form. At least three separate fragments from the terminal region were able to inhibit chloramphenicol acetyltransferase expression when inserted between either the PARP, the ribosomal, or the variable surface glycoprotein promoter and a chloramphenicol acetyltransferase reporter gene. This inhibition was due to an orientation-dependent transcription termination caused by the combination of several attenuator elements with no obvious sequence conservation. The procyclin transcription terminator appeared unable to inhibit transcription by polymerase II.

摘要

布氏锥虫的多顺反子前环素PARP（前环酸性重复蛋白）转录单元通过终止区域的定位得到了完整的表征。除了前环素基因串联和GRESAG 2.1外，这个7.5至9.5 kb的单元还包含另一个假定表面蛋白的基因，称为PAG（前环素相关基因）3。末端3 kb序列不包含明显的开放阅读框，并且与血流形式特有的一个或几个转录单元的起始部分交叉杂交。当从末端区域分离的至少三个片段插入PARP、核糖体或可变表面糖蛋白启动子与氯霉素乙酰转移酶报告基因之间时，它们能够抑制氯霉素乙酰转移酶的表达。这种抑制是由于几种衰减元件组合导致的方向依赖性转录终止，这些衰减元件没有明显的序列保守性。前环素转录终止子似乎无法抑制聚合酶II的转录。