Rudenko G, Le Blancq S, Smith J, Lee M G, Rattray A, Van der Ploeg L H
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Mol Cell Biol. 1990 Jul;10(7):3492-504. doi: 10.1128/mcb.10.7.3492-3504.1990.
At least one of the procyclic acidic repetitive protein (PARP or procyclin) loci of Trypanosoma brucei is a small (5- to 6-kilobase) polycistronic transcription unit which is transcribed in an alpha-amanitin-resistant manner. Its single promoter, as mapped by run-on transcription analysis and UV inactivation of transcription, is located immediately upstream of the first alpha-PARP gene. Transcription termination occurs in a region approximately 3 kilobases downstream of the beta-PARP gene. The location of the promoter was confirmed by its ability to direct transcription of the bacterial chloramphenicol acetyltransferase gene in insect-form (procyclic) T. brucei. The putative PARP promoter is located in the region between the 3' splice acceptor site (nucleotide position 0) and nucleotide position -196 upstream of the alpha-PARP genes. Regulatory regions influencing the levels of PARP expression may be located further upstream. We conclude that a single promoter, which is located very close to the 3' splice acceptor site of the alpha-PARP genes, directs the transcription of a small, polycistronic, and alpha-amanitin-resistant transcription unit.
布氏锥虫的至少一个前循环酸性重复蛋白（PARP或前循环素）基因座是一个小的（5至6千碱基）多顺反子转录单元，它以对α-鹅膏蕈碱抗性的方式进行转录。通过连续转录分析和转录的紫外线失活确定的其单一启动子，位于第一个α-PARP基因的紧邻上游。转录终止发生在β-PARP基因下游约3千碱基的区域。该启动子的位置通过其在昆虫形式（前循环）布氏锥虫中指导细菌氯霉素乙酰转移酶基因转录的能力得以证实。推定的PARP启动子位于α-PARP基因的3'剪接受体位点（核苷酸位置0）和上游-196核苷酸位置之间的区域。影响PARP表达水平的调控区域可能位于更上游。我们得出结论，一个非常靠近α-PARP基因3'剪接受体位点的单一启动子指导一个小的、多顺反子的、对α-鹅膏蕈碱抗性的转录单元的转录。
