Orozco J, Mendel R C, Hahn M R, Guthkelch A N, Carter L P
University of Arizona, Health Sciences Center, Tucson, USA.
Neurol Res. 1995 Dec;17(6):423-5.
This study was designed to evaluate the effects of tissue-type plasminogen activator (tPA) and 21- amino steroid (U74006F) in experimental embolic stroke in rabbits. The size of infarction from embolism was compared to controls with tPA alone, 21-amino steroid alone, and in combination. The middle cerebral artery of the rabbit was embolized by injecting an arterial ('white') thrombus in the right internal carotid artery. The rabbit treatment was 2 mg kg-1 of tissue-type plasminogen activator and/or 3 mg kg-1 of 21 amino steroid started at 2 h post-embolization. The animals were terminated 4 h post-treatment and brains were examined for evidence of ischemia and/or hemorrhage. Administration of tissue-type plasminogen activator and/or 21-amino steroid in the raw data show that there is a tendency for all treatments to reduce the ischemic volume when compared to the control group, also it is evident the standard deviation of these estimates is rather large when compared to the differences between treatments. The results of the analysis of variance shows that the differences expressed are not statistically significant. (No statistical differences were found between the treatment groups and the control group.) The results show that administration of tissue-type plasminogen activator and/or 21 amino steroid at 2 h post-embolization alone or in simultaneous administration does not significantly reduce the volume of infarction. Further studies need to be addressed in regards to the region of viable brain in the peri-infarct area, in reducing the time to treatment.
本研究旨在评估组织型纤溶酶原激活剂（tPA）和21-氨基类固醇（U74006F）对兔实验性栓塞性中风的影响。将栓塞所致梗死灶大小与单独使用tPA、单独使用21-氨基类固醇以及联合使用的对照组进行比较。通过向兔右侧颈内动脉注射动脉（“白色”）血栓来栓塞大脑中动脉。兔的治疗方案为在栓塞后2小时开始给予2mg/kg的组织型纤溶酶原激活剂和/或3mg/kg的21-氨基类固醇。在治疗后4小时处死动物，并检查大脑有无缺血和/或出血迹象。原始数据中组织型纤溶酶原激活剂和/或21-氨基类固醇的给药情况表明，与对照组相比，所有治疗组均有减少缺血体积的趋势，而且与各治疗组之间的差异相比，这些估计值的标准差相当大。方差分析结果表明，所表达的差异无统计学意义。（治疗组与对照组之间未发现统计学差异。）结果表明，在栓塞后2小时单独或同时给予组织型纤溶酶原激活剂和/或21-氨基类固醇并不能显著减少梗死体积。关于梗死周边存活脑组织区域以及缩短治疗时间，还需要进一步研究。
