Kraly F S, Tribuzio R A, Kim Y M, Keefe M E, Finkell J
Department of Psychology, Colgate University, Hamilton, NY 13346, USA.
Physiol Behav. 1995 Dec;58(6):1091-7. doi: 10.1016/0031-9384(95)02048-9.
A role for endogenous histamine and its H3 receptor subtype for mediating drinking elicited by eating was examined in adult male Sprague-Dawley rats. The i.p. injection of the H3 agonist R-alpha-methylhistamine (Ramh, 2.5 mg/kg) shortened the latency to initiate drinking and increased 1-h water intake in nondeprived rats freely eating pellets and drinking water. The ICV injection (through a surgically implanted chronic cannula) of 10 micrograms Ramh increased water intake; this Ramh-induced drinking was abolished by previous ICV injection of the H3 antagonist thioperamide (Th, 60 micrograms). For rats drinking and eating after 24-h food deprivation, s.c. Th inhibited drinking behavior: for example, 10 mg/kg Th s.c. delayed the latency to initiate drinking and inhibited 1-h water intake without inhibition of food intake. In contrast, 60 micrograms Th ICV failed to inhibit food-related drinking in rats eating after food deprivation. For nondeprived rats eating a small cracker, 10 mg/kg Th s.c. delayed the latency to initiate drinking and abolished water intake without effect of eating, and 60 micrograms Th ICV had similar effects upon drinking elicited by ingestion of cracker. The IG infusion (through a surgically implanted gastric catheter) of 2 ml 600 or 900 mOsm/kg NaCl, a treatment that is subthreshold for increase in systemic plasma osmolality at the initiation of drinking, elicited drinking that was abolished by 10 mg/kg Th s.c. and attenuated by 60 micrograms Th ICV. The IG infusion of 2 ml 1800 mOsm/kg NaCl, a treatment that is above threshold for increase in systemic plasma osmolality, elicited drinking that was attenuated by 10 mg/kg s.c. or 60 micrograms Th ICV. These results demonstrate that peripheral and central H3 receptors for histamine have a role in drinking elicited by eating and the postprandial gastrointestinal osmotic consequences of eating. These findings extend the evidence demonstrating a histaminergic contribution to food-related drinking in rats.
在成年雄性Sprague-Dawley大鼠中，研究了内源性组胺及其H3受体亚型在介导进食引起的饮水方面的作用。腹腔注射H3激动剂R-α-甲基组胺（Ramh，2.5毫克/千克）缩短了非剥夺大鼠自由进食颗粒饲料和饮水时开始饮水的潜伏期，并增加了1小时的饮水量。通过手术植入的慢性套管进行脑室内注射10微克Ramh可增加饮水量；先前脑室内注射H3拮抗剂硫代哌啶（Th，60微克）可消除这种由Ramh诱导的饮水。对于24小时食物剥夺后饮水和进食的大鼠，皮下注射Th可抑制饮水行为：例如，皮下注射10毫克/千克Th可延迟开始饮水的潜伏期并抑制1小时的饮水量，而不抑制食物摄入量。相比之下，脑室内注射60微克Th未能抑制食物剥夺后进食大鼠与食物相关的饮水。对于非剥夺大鼠食用小饼干的情况，皮下注射10毫克/千克Th可延迟开始饮水的潜伏期并消除饮水量，而对进食无影响，脑室内注射60微克Th对食用饼干引起的饮水有类似影响。通过手术植入的胃导管进行胃内输注2毫升600或900毫渗摩尔/千克NaCl，这种处理在饮水开始时对全身血浆渗透压升高低于阈值，可引起饮水，皮下注射10毫克/千克Th可消除这种饮水，脑室内注射60微克Th可使其减弱。胃内输注2毫升1800毫渗摩尔/千克NaCl，这种处理高于全身血浆渗透压升高的阈值，可引起饮水，皮下注射10毫克/千克或脑室内注射60微克Th可使其减弱。这些结果表明，组胺的外周和中枢H3受体在进食引起的饮水以及进食后胃肠道渗透后果方面发挥作用。这些发现扩展了表明组胺能对大鼠与食物相关饮水有贡献的证据。
