通过聚合酶链反应进行镰刀菌全眼球炎的尸检诊断。
Postmortem diagnosis of Fusarium panophthalmitis by the polymerase chain reaction.
作者信息
Alexandrakis G, Sears M, Gloor P
机构信息
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06520, USA.
出版信息
Am J Ophthalmol. 1996 Feb;121(2):221-3. doi: 10.1016/s0002-9394(14)70594-x.
PURPOSE
We undertook this study to determine if a polymerase chain reaction-based test that we developed for the filamentary fungus, Fusarium, could be used to detect the organism in postmortem ocular tissues.
METHODS
We applied the polymerase chain reaction to amplify a target fragment of Fusarium DNA from formalin-fixed ocular tissues from a patient with endogenous Fusarium panophthalmitis.
RESULTS
By using the polymerase chain reaction-based test, we were able to amplify the target fragment of DNA from the infected eyes, but not from uninfected control eyes.
CONCLUSIONS
The technique appears to hold promise to be a sensitive, specific, and rapid method of diagnosing Fusarium infections.
目的
我们开展这项研究以确定我们开发的用于丝状真菌镰刀菌的基于聚合酶链反应的检测方法是否可用于检测死后眼组织中的该生物体。
方法
我们应用聚合酶链反应从一名患有内源性镰刀菌全眼球炎患者的福尔马林固定眼组织中扩增镰刀菌DNA的目标片段。
结果
通过使用基于聚合酶链反应的检测方法，我们能够从受感染的眼睛中扩增出DNA目标片段，但未从未受感染的对照眼中扩增出。
结论
该技术似乎有望成为一种诊断镰刀菌感染的灵敏、特异且快速的方法。
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