Foley R C, Liang Z M, Singh K B
Department of Molecular, Cell and Developmental Biology, University of California Los Angeles 90095-1606, USA.
Plant Mol Biol. 1996 Feb;30(4):687-95. doi: 10.1007/BF00019004.
We have isolated a gene from bean (Vicia faba L.), called Vein1, that encodes a novel protein. The Vein1 cDNA was isolated as a result of a differential screen for genes that are expressed in leaves but not in the most common cell type, the mesophyll cell. Northern blot analysis revealed that Vein 1 transcripts are differentially expressed in the plant with expression in leaves, stems and sepals but not in petals, mesophyll cells or roots. In situ hybridization studies of stem and leaf sections indicate that the expression of Vein1 is localized to the phloem tissue. Interestingly, Vein1 was differentially expressed in stem tissue with the highest expression in the oldest internodes. The deduced Vein1 protein sequence does not share homology with any known protein sequences. The 17 kDa Vein1 protein is highly hydrophilic and contains a histidine-rich motif, where six out of seven amino acids are histidines. The function of Vein1 is unknown, although the expression patterns suggests that it may play a role in mature phloem tissue in the aerial parts of the plant.
我们从蚕豆（Vicia faba L.）中分离出一个名为Vein1的基因，它编码一种新型蛋白质。Vein1 cDNA是通过对在叶片中表达但不在最常见细胞类型即叶肉细胞中表达的基因进行差异筛选而分离得到的。Northern印迹分析表明，Vein 1转录本在植物中差异表达，在叶、茎和萼片中表达，但在花瓣、叶肉细胞或根中不表达。对茎和叶切片的原位杂交研究表明，Vein1的表达定位于韧皮部组织。有趣的是，Vein1在茎组织中差异表达，在最老的节间中表达最高。推导的Vein1蛋白序列与任何已知蛋白序列均无同源性。17 kDa的Vein1蛋白具有高度亲水性，包含一个富含组氨酸的基序，其中七个氨基酸中有六个是组氨酸。尽管表达模式表明Vein1可能在植物地上部分的成熟韧皮部组织中发挥作用，但其功能尚不清楚。
