van der Kop D A, Schuyer M, Scheres B, van der Zaal B J, Hooykaas P J
Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
Plant Mol Biol. 1996 Feb;30(4):839-44. doi: 10.1007/BF00019016.
Genes homologous to the auxin-inducible Nt103 glutathione S-transferase (GST) gene of tobacco, were isolated from a genomic library of Arabidopsis thaliana. We isolated a lambda clone containing an auxin-inducible gene, At103-1a, and part of a constitutively expressed gene, At103-1b. The coding regions of the Arabidopsis genes were highly homologous to each other and to the coding region of the tobacco gene but distinct from the GST genes that have been isolated from arabidopsis thusfar. Overexpression of a cDNA clone in Escherichia coli revealed that the AT103-1A protein had GST activity.
从拟南芥基因组文库中分离出了与烟草生长素诱导型Nt103谷胱甘肽S-转移酶（GST）基因同源的基因。我们分离出了一个λ克隆，其中包含一个生长素诱导型基因At103-1a和一个组成型表达基因At103-1b的一部分。拟南芥基因的编码区彼此高度同源，也与烟草基因的编码区高度同源，但与迄今为止从拟南芥中分离出的GST基因不同。在大肠杆菌中过表达一个cDNA克隆表明，AT103-1A蛋白具有GST活性。
