Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
FEBS Lett. 1993 Dec 6;335(2):189-92. doi: 10.1016/0014-5793(93)80727-c.
Two cDNA clones, designated ERD11 and ERD13, isolated from a cDNA library from Arabidopsis thaliana L. plants dehydrated for 1 h were sequenced and characterized. These clones encoded polypeptides that were homologous to glutathione S-transferases of tobacco and maize. Genomic Southern hybridization suggested that there are a few additional genes showing high similarity to the ERD11 gene in the Arabidopsis genome. The expression of the genes for ERD11 and ERD13 was induced by dehydration, but was not affected by the application of four plant growth regulators, 2,4-dichlorophenoxyacetic acid 6-benzylaminopurine, abscisic acid, or gibberellic acid.
从脱水1小时的拟南芥植株的cDNA文库中分离出两个cDNA克隆，分别命名为ERD11和ERD13，并对其进行了测序和特征分析。这些克隆编码的多肽与烟草和玉米的谷胱甘肽S-转移酶同源。基因组Southern杂交表明，拟南芥基因组中还有一些与ERD11基因高度相似的其他基因。ERD11和ERD13基因的表达受脱水诱导，但不受四种植物生长调节剂（2,4-二氯苯氧乙酸、6-苄基腺嘌呤、脱落酸或赤霉素）处理的影响。
