两性霉素B对大鼠心脏肌浆网膜ATP酶活性的体外抑制作用及其被1,6 - 二磷酸果糖逆转的作用
In vitro inhibition of rat heart sarcoplasmic reticular membrane ATPase activities by amphotericin B and their reversal by fructose-1,6-diphosphate.
作者信息
Rao M R, Hutcheson A E, Markov A K
机构信息
Department of Medicine, University of Mississippi Medical Center, Jackson 39216, USA.
出版信息
Biochem Mol Biol Int. 1995 Nov;37(5):821-5.
The effect of amphotericin B on rat heart sarcoplasmic reticular membrane Na(+)-K+ and Ca2+ ATPase activities in vitro was investigated. Amphotericin B in selected concentrations of 100-1000 ng significantly inhibited the sarcoplasmic reticular membrane ATPase activities studied. Fructose-1,6-diphosphate (1000 microM concentration) completely reversed the inhibition of Ca2+ ATPase activity in particular, but failed to reverse that of Na+(-)K+ATPase activities at 1000 microM concentration. Fructose-1,6-diphosphate may afford some protection against 1000 ng amphotericin B-induced myocardial toxicity. These damages may vary depending upon the dose of amphotericin B used in experimental studies.
研究了两性霉素B对大鼠心脏肌浆网膜Na(+)-K+和Ca2+ ATP酶活性的体外作用。选定浓度为100 - 1000 ng的两性霉素B显著抑制了所研究的肌浆网膜ATP酶活性。尤其是1000微摩尔浓度的1,6-二磷酸果糖完全逆转了Ca2+ ATP酶活性的抑制作用，但在1000微摩尔浓度时未能逆转Na+(-)K+ATP酶活性的抑制作用。1,6-二磷酸果糖可能对1000 ng两性霉素B诱导的心肌毒性提供一定的保护作用。这些损伤可能因实验研究中使用的两性霉素B剂量不同而有所差异。
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