Rao M R, Olinde K D, Markov A K
Department of Medicine, The University of Mississippi School of Medicine, Jackson 39216, USA.
Mol Cell Biochem. 1998 Aug;185(1-2):171-5. doi: 10.1023/a:1006840802595.
Nitric oxide (NO) functions as a cellular messenger in a number of organs and cell systems in the cardiovascular system (CVS); it is a significant determinant of basal vascular tone and regulates myocardial contractility and platelet aggregation. The present study focused upon understanding the in vitro effects of fructose-1,6-diphosphate (FDP) on the rat cellular NO pathway. The iNOS activity was measured by monitoring the formation of (3H)-citrulline in 50,000 g soluble fractions of crude homogenates of endothelial (ET) and smooth muscle cells (SMC) from the arteries of rats, and macrophages (MAC) and lymphocytes (LYM) from rat blood. FDP in concentrations of 10-1000 microM stimulated rat cellular iNOS activity in a concentration-dependent manner. FDP-stimulated rat cellular iNOS was found to be completely reversed by 5 microM concentration of NG-monomethyl-L-arginine (L-NMMA), the potent mammalian NOS inhibitor. These studies demonstrated that FDP may induce the formation of NO by stimulating rat cardiovascular iNOS activity.
一氧化氮（NO）在心血管系统（CVS）的许多器官和细胞系统中作为细胞信使发挥作用；它是基础血管张力的重要决定因素，并调节心肌收缩力和血小板聚集。本研究着重于了解1,6-二磷酸果糖（FDP）对大鼠细胞NO途径的体外影响。通过监测大鼠动脉内皮细胞（ET）和平滑肌细胞（SMC）以及大鼠血液中的巨噬细胞（MAC）和淋巴细胞（LYM）的50,000g粗匀浆可溶性部分中（3H）-瓜氨酸的形成来测量诱导型一氧化氮合酶（iNOS）活性。浓度为10 - 1000微摩尔的FDP以浓度依赖性方式刺激大鼠细胞iNOS活性。发现5微摩尔浓度的NG-单甲基-L-精氨酸（L-NMMA）（一种有效的哺乳动物NOS抑制剂）可完全逆转FDP刺激的大鼠细胞iNOS。这些研究表明，FDP可能通过刺激大鼠心血管iNOS活性诱导NO的形成。
