Tiwari V, Jain A, Agarwal M, Mittal B, Pandey H P
Department of Microbiology, King George's Medical College, Lucknow, India.
Biochem Mol Biol Int. 1995 Nov;37(5):959-64.
When infected with Leishmania species, patients develop specific antibodies that constitute the basis of serodiagnosis. using Western blot analysis we studied the specificity of anti-leishmania donovani antibodies in patients with visceral leishmaniasis, healthy subjects living in an endemic and non-endemic areas, and patients of other infectious diseases like malaria, leprosy, tuberculosis and tropical splenomegaly. Sera from patients with kala-azar recognised numerous antigens that had a molecular weight of 150 KD, 145 KD, 120 KD, 92 KD, 87 KD, 72 KD, 65 KD, 56 KD, 50 KD, 40 KD, 26 KD, 21 KD, 14 KD, AND 12 KD. The 150, 145, 120, 92, 87, 81, 65, 25, 21, 14, and 12 KD antigens had the greatest specificity for kala-azar sera while the bands of molecular weights 72, 56, 50, and 40 KD were found to be cross reactive with sera of patients of other diseases.
感染利什曼原虫属时，患者会产生构成血清学诊断基础的特异性抗体。我们采用蛋白质印迹分析研究了内脏利什曼病患者、生活在流行区和非流行区的健康受试者以及疟疾、麻风、结核病和热带脾肿大等其他传染病患者体内抗杜氏利什曼原虫抗体的特异性。黑热病患者的血清识别出许多分子量为150 KD、145 KD、120 KD、92 KD、87 KD、72 KD、65 KD、56 KD、50 KD、40 KD、26 KD、21 KD、14 KD和12 KD的抗原。150、145、120、92、87、81、65、25、21、14和12 KD抗原对黑热病血清具有最大特异性，而分子量为72、56、50和40 KD的条带被发现与其他疾病患者的血清有交叉反应。
