通过表达克隆以及用来自印度比哈尔邦黑热病患者血清进行免疫印迹法鉴定内脏利什曼病中的新抗原
Identification of new antigens in visceral leishmaniasis by expression cloning and immunoblotting with sera of kala-azar patients from Bihar, India.
作者信息
Theinert Stephan M, Basu Rajatava, Forgber Michael, Roy Syamal, Sundar Shyam, Walden Peter
机构信息
Clinical Research Group, Department of Dermatology, Charité University Medicine Berlin, Humboldt University, Germany.
出版信息
Infect Immun. 2005 Oct;73(10):7018-21. doi: 10.1128/IAI.73.10.7018-7021.2005.
Abstract
Sera of kala-azar patients from Bihar, India, were used to identify Leishmania donovani antigens encoded by a phage expression library. Ten antigens were identified, five of which have not been described as leishmania antigens before. The antigens specifically react with sera of leishmania-infected patients but not of toxoplasma- or plasmodium-infected patients.
摘要
来自印度比哈尔邦的黑热病患者血清被用于鉴定由噬菌体表达文库编码的杜氏利什曼原虫抗原。鉴定出了10种抗原，其中5种以前未被描述为利什曼原虫抗原。这些抗原与利什曼原虫感染患者的血清特异性反应，但与弓形虫或疟原虫感染患者的血清不发生反应。
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