Koga Y, Kuwano H, Sugimachi K
Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer. 1996 Jan 15;77(2):237-44. doi: 10.1002/(SICI)1097-0142(19960115)77:2<237::AID-CNCR3>3.0.CO;2-J.
Proliferating cell nuclear antigen (PCNA) correlates with the cell proliferative state. Using a PCNA specific monoclonal antibody, various malignant neoplasms have been assessed for cell proliferation and malignant potential. However, there are few studies regarding the expression of PCNA in esophageal dysplasia.
PCNA expression was immunohistochemically determined in resected specimens from 27 patients with superficial esophageal carcinoma that contained areas with various degrees of dysplasia, carcinoma in situ (CIS), and carcinoma invading the mucosal layer. The authors estimated the cell proliferative activity and biologic characteristics of the basal and parabasal cells of these esophageal epithelial disorders.
The degrees of PCNA labeling index (PCNA LI) of the basal layers were lower than those of the parabasal layers in normal epithelium and dysplastic lesions, respectively, whereas no difference in CIS was recognized between the two layers. The proportion of PCNA LI in the basal layer of moderate dysplasia was lower than that in the basal layer of severe dysplasia. Conversely, there were no statistical differences between severe dysplasia and CIS in both basal and parabasal layers. In terms of lymphocytic infiltration beneath the dysplasia, there was a close relationship between the degree of lymphocytic infiltration and the PCNA index of the basal and parabasal layers.
PCNA expression may be a useful marker to reflect the biologic characteristics of esophageal dysplasia, suggesting that a high grade of dysplasia is as serious a lesion as esophageal carcinoma.
增殖细胞核抗原（PCNA）与细胞增殖状态相关。使用PCNA特异性单克隆抗体，已对各种恶性肿瘤的细胞增殖和恶性潜能进行了评估。然而，关于PCNA在食管发育异常中的表达的研究较少。
对27例浅表性食管癌切除标本进行免疫组织化学检测PCNA表达，这些标本包含不同程度发育异常、原位癌（CIS）和侵犯黏膜层的癌灶区域。作者评估了这些食管上皮病变的基底细胞和副基底细胞的细胞增殖活性及生物学特性。
在正常上皮和发育异常病变中，基底细胞层的PCNA标记指数（PCNA LI）分别低于副基底细胞层，而在原位癌中两层之间未发现差异。中度发育异常基底细胞层的PCNA LI比例低于重度发育异常基底细胞层。相反，重度发育异常和原位癌在基底细胞层和副基底细胞层均无统计学差异。就发育异常下方的淋巴细胞浸润而言，淋巴细胞浸润程度与基底细胞层和副基底细胞层的PCNA指数之间存在密切关系。
PCNA表达可能是反映食管发育异常生物学特性的有用标志物，提示高度发育异常与食管癌一样是严重病变。
