Volpi E V, Vatcheva R, Labella T, Gan S U
Istituto di Genetica Molecolare del CNR, Alghero, Italy.
Cancer Genet Cytogenet. 1996 Apr;87(2):103-6. doi: 10.1016/0165-4608(95)00214-6.
We performed a focused chromosome analysis on the HL60 cell line by multicolor fluorescence in situ hybridization (FISH), using probes for the unequivocal identification of specific chromosome regions and subregions. The purpose of this karyotypic re-evaluation was to confirm and to characterize in more detail chromosome rearrangements already identified by means of classic cytogenetic approaches and recurrently detected from the initial establishment of the cell line. The observations reported may help reassess the potential of the HL60 cell line in understanding the molecular events underlying the non-random karyotype alterations associated with acute myeloid leukemias (AML).
我们通过多色荧光原位杂交（FISH）对HL60细胞系进行了针对性的染色体分析，使用探针明确鉴定特定的染色体区域和亚区域。此次核型重新评估的目的是确认并更详细地描述已通过经典细胞遗传学方法鉴定且在该细胞系最初建立时反复检测到的染色体重排。所报告的观察结果可能有助于重新评估HL60细胞系在理解与急性髓系白血病（AML）相关的非随机核型改变背后分子事件方面的潜力。
